Activity of Tea Tree (Melaleuca alternifolia) Essential Oil against L3 Larvae of Anisakis simplex
Activity of Tea Tree (Melaleuca alternifolia) Essential Oil against L3 Larvae of Anisakis simplex
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DOI：
10.1155/2014/549510
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Lopez, Victor
中科院分区：
文献类型：
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作者：
Gomez-Rincon, Carlota;Langa, Elisa;Lopez, Victor
研究了互花千层精油（俗称茶树油）对单纯异尖线虫L3幼虫的体外杀线虫活性。结果表明，浓度在7 ~ 10 μ L/mL之间，48h后死亡率为100%，24h后LD50值为4.53 μ L/mL， 48h后LD50值为4.27 μ L/mL。茶树精油对乙酰胆碱酯酶的抑制呈浓度依赖性，在100 μ L/mL时抑制值为100%。这一事实表明，TTO可能起AChE抑制剂的作用。由于未表现出杀幼虫或抗胆碱酯酶活性，因此将松油烯-4-醇作为主要杀幼虫化合物丢弃。结果表明，互花千层精油对治疗人类异丝虫病具有很大的治疗潜力。
Nematicidal activity of Melaleuca alternifolia essential oil, commonly known as tea tree oil (TTO), was assayed in vitro against L3 larvae of Anisakis simplex. The results showed a mortality of 100% for concentrations between 7 and 10 mu L/mL after 48 h of incubation, obtaining an LD50 value of 4.53 mu L/mL after 24 hours and 4.27 mu L/mL after 48 hours. Concentration-dependent inhibition of acetylcholinesterase was observed for tea tree essential oil showing inhibition values of 100% at 100 mu L/mL. This fact suggests that TTO may act as an AChE inhibitor. Terpinen-4-ol was discarded as main larvicide compound as it did not show larvicidal or anticholinesterase activity. The data obtained suggest that the essential oil of Melaleuca alternifolia may have a great therapeutic potential for the treatment of human anisakiasis.