THE SEPARATE ROLES OF PLANT CIS AND TRANS PRENYL TRANSFERASES IN CIS-1,4-POLYISOPRENE BIOSYNTHESIS
THE SEPARATE ROLES OF PLANT CIS AND TRANS PRENYL TRANSFERASES IN CIS-1,4-POLYISOPRENE BIOSYNTHESIS
复制标题
DOI：
10.1111/j.1432-1033.1993.tb18374.x
复制
发表时间：
1993-11-15
期刊：
影响因子：
--
通讯作者：
CORNISH, K
中科院分区：
文献类型：
--
作者：
CORNISH, K
在植物中，顺式-1，4-聚异戊二烯（天然橡胶，M（r）> 10（6））的伸长需要少量的反式烯丙基二磷酸（小于或等于C20）引发剂。反式烯丙基二磷酸是亲水性的胞质化合物，而顺式-1，4-聚异戊二烯是疏水性的，并在亚细胞rubber particles.In本文中，证明了可溶性trans-prenyl转移酶从橡胶胶乳的功能仅作为法呢基二磷酸合成酶，并没有发挥直接作用的顺式-1，4-聚异戊二烯的延伸。顺式-1，4-异戊二烯基转移酶与H. brasiliensis橡胶颗粒，在其他产橡胶物种中也是如此[Archer，B. L.，奥德利，B。G.，Cockbain，E. G. & McSweeney，G.（1963）Biochem.J.89，565-574; Madhavan，S.，格林布拉特湾一、福斯特，M. A. & Benedict，C. R.（1989）Plant Physiol.89，506 -511; Siler，D. J. &康沃尔语，K.（1993）Phytochemistry 32，1097-1102]。实验数据解释和反驳了以前的报道，其中可溶性的trans-prenyl转移酶分离自H。brasiliensis胶乳具有反式异戊二烯基转移酶和顺式异戊二烯基转移酶活性[Light，D. R. & Dennis，M. S.（1989）J.Biol.Chem.264，18589 -18597; Light，D. R.，拉撒路河，巴西-地A. & Dennis，M. S.（1989）J.Biol.Chem.264，18598 -18607]。因此，似乎植物异戊二烯基转移酶与动物酶系统相当，其中反式异戊二烯基转移酶是可溶性酶而顺式异戊二烯基转移酶是膜结合的[Ericcson，J.，Runquist，M.，Thelin，A.，Andersson，M.，Chojnacki，T. & Dallner，G.（1992）J.BiolChem.268，832 -838]。
In plants, the elongation of cis-1,4-polyisoprene (natural rubber, M(r) > 10(6)) requires a small trans-allylic diphosphate (less-than-or-equal-to C20) initiator. The trans-allylic diphosphates are hydrophilic cytosolic compounds, whereas cis-1,4-polyisoprene is hydrophobic and compartmentalised in subcellular rubber particles.In this paper, it is demonstrated that soluble trans-prenyl transferase from latex of Hevea brasiliensis functions solely as farnesyl diphosphate synthase, and plays no direct role in cis-1,4-polyisoprene elongation. The cis-1,4-prenyl transferase is firmly associated with the H. brasiliensis rubber particle, as is also the case in other rubber-producing species [Archer, B. L., Audley, B. G., Cockbain, E. G. & McSweeney, G. P. (1963) Biochem. J. 89, 565-574; Madhavan, S., Greenblatt, G. A., Foster, M. A. & Benedict, C. R. (1989) Plant Physiol. 89,506-511; Siler, D. J. & Cornish, K. (1993) Phytochemistry 32, 1097-1102]. The experimental data explain and refute previous reports in which soluble trans-prenyl transferase isolated from H. brasiliensis latex was attributed both trans-prenyl transferase and cis-prenyl transferase activities [Light, D. R. & Dennis, M. S. (1989) J. Biol. Chem. 264,18589-18597; Light, D. R., Lazarus, R. A. & Dennis, M. S. (1989) J. Biol. Chem. 264,18598-18607].Thus, it appears that plant prenyl transferases are comparable to animal enzyme systems in which trans-prenyl transferases are soluble enzymes whilst cis-prenyl transferases are membrane-bound [Ericcson, J., Runquist, M., Thelin, A., Andersson, M., Chojnacki, T. & Dallner, G. (1992) J. Biol Chem. 268,832-838].