Study on the cytotoxicity of microcystin-LR on cultured cells
Study on the cytotoxicity of microcystin-LR on cultured cells
复制标题
DOI：
10.1016/s0045-6535(99)00402-6
复制
发表时间：
2000-07-01
期刊：
影响因子：
8.8
通讯作者：
Fong, WF
中科院分区：
文献类型：
--
作者：
Chong, MWK;Gu, KD;Fong, WF
研究了纯化的蓝藻毒素-微囊藻毒素-LR对KB、NIH/3 T3、H-4-II-E、HeLa、Vero、Hep G2、Caco-2和HL-60等细胞株的毒性。用比色法3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴盐（MTT）测定鳗鱼存活率的评估表明，在毒素浓度大于18.75 μ g/ml的情况下孵育96小时后，纯化的微囊藻毒素-LR对KB和H-4-II-E细胞系诱导毒性作用。考虑到重复性、一致性和敏感性，选择KB细胞系进行进一步研究。当毒素浓度为18.75 μ g/ml时，孵育时间为72和96 h时，RE细胞释放出大量的乳酸脱氢酶（LDH）。和更高。虽然以前的研究表明，微囊藻毒素-LR对永久性细胞株没有细胞毒性作用，但KB细胞的LDH释放试验表明，暴露于微囊藻毒素-LR可导致细胞膜损伤。(C)2000爱思唯尔科技有限公司版权所有。
The toxicity of purified blue-green algal toxin, microcystin-LR, on permanent cell lines KB, NIH/3T3, H-4-II-E, HeLa, Vero, Hep G2, Caco-2 and HL-60 was studied. Assessment of eel viability using colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays indicated that purified microcystin-LR induced toxic effect on KB and H-4-II-E cell lines after 96 h incubation at toxin concentrations greater than 18.75 mu g/ml. KB cell line was selected for further study when reproducibility, consistency and sensitivity were considered. Significant amounts of lactate dehydrogenase (LDH) were released from RE cells when incubation durations were 72 and 96 h with toxin concentrations of 18.75 mu g/ml. and higher. Although previous studies suggested that microcystin-LR had no cytotoxic effect on permanent cell lines, LDH release assay performed on KB cells indicated that exposure to microcystin-LR could result in damage to the cell membrane. (C) 2000 Elsevier Science Ltd. All rights reserved.