The Adjuvant Use of Stromal Vascular Fraction and Platelet-Rich Fibrin for Autologous Adipose Tissue Transplantation
The Adjuvant Use of Stromal Vascular Fraction and Platelet-Rich Fibrin for Autologous Adipose Tissue Transplantation
复制标题
基质血管成分和富含血小板的纤维蛋白在自体脂肪组织移植中的辅助应用
DOI：
10.1089/ten.tec.2012.0126
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发表时间：
2013-01-01
影响因子：
3
通讯作者：
Chen, Fa-Ming
中科院分区：
文献类型：
--
作者：
Liu, Bin;Tan, Xin-Ying;Chen, Fa-Ming
自体脂肪移植在修复软组织缺损和脂肪萎缩以及改善美观方面（例如，面部重建和年轻化）。然而，目前的技术是粗糙的，具有严重的缺点，特别是由于移植的脂肪组织的再吸收和有限的脂肪形成而导致的体积维持的长期不可预测性。我们假设辅助使用患者来源的脂肪基质血管成分（SVF）和富血小板纤维蛋白（PRF）可能会提高整形和重建手术中自体脂肪移植的总体结果。在手术使用前，制备了平均细胞数为（4.8 +/- 3.79）x 10（7）个细胞/mL、平均细胞活力为71.8%的自体SVF和持续释放多种血管生成生长因子的自体PRF。根据以下研究设计，将以下脂肪组织植入物皮下注射到兔耳的耳廓中：2 mL脂肪颗粒和0.2 mL生理盐水溶液AG + NS组：2 mL脂肪颗粒和0.2mL SVF（AG + SVF组）、2 mL脂肪颗粒+0.2mL PRF（AG + PRF组）或2 mL脂肪颗粒+0.1mL SVF +0.1mL PRF（AG + SVF + PRF组）。组织学检查显示，AG + SVF + PRF组植入的脂肪颗粒移植良好，植入后4周微血管密度明显高于其他3组（p < 0.01）。植入后24周，各组植入组织的吸收率分别为49.39% ± 9.47%、27.25% ± 4.37%、36.41% ± 8.47%和17.37% ± 6.22%，差异有显著性（p < 0.01）。结果表明，使用自体PRF和SVF作为治疗佐剂可以提高脂肪组织植入的疗效，为软组织增强和重建提供了一种临床可转化的策略。
Autologous adipose transplantation is rapidly gaining popularity for the restoration of soft tissue defects and lipoatrophy as well as for aesthetic improvements (e.g., facial reconstruction and rejuvenation). However, the current technique is crude that suffers from serious demerits, particularly the long-term unpredictability of volume maintenance due to resorption of the grafted adipose tissue and limited adipogenesis. We hypothesized that the adjuvant use of patient-derived adipose stromal vascular fraction (SVF) and platelet-rich fibrin (PRF) may enhance the overall outcome of autologous fat grafting in plastic and reconstructive surgery. Autologous SVF, with a mean cell number of (4.8 +/- 3.79) x 10(7) cells/mL and a mean cell viability of 71.8%, and autologous PRF, with sustained release of multiple angiogenic growth factors, were created before surgical use. The following adipose tissue implants were injected subcutaneously into a rabbit ear's auricula according to the following study design: 2 mL adipose granules and 0.2 mL normal saline solution (AG + NS group), 2 mL adipose granules and 0.2 mL SVF (AG + SVF group), 2 mL adipose granules and 0.2 mL PRF (AG + PRF group), or 2 mL adipose granules combined with 0.1 mL SVF and 0.1 mL PRF (AG + SVF + PRF group). Histological examinations showed that the implanted adipose granules were well engrafted in the AG + SVF + PRF group, with a higher microvessel density 4 weeks postimplantation compared with the other three groups (p < 0.01). Twenty-four weeks postimplantation, the resorption rates of implanted tissue in each group were 49.39% +/- 9.47%, 27.25% +/- 4.37%, 36.41% +/- 8.47%, and 17.37% +/- 6.22%, respectively, and were significantly different (p < 0.01). The results demonstrated that the efficacy of adipose tissue implantation can be enhanced by using autologous PRF and SVF as therapeutic adjuvants, offering a clinically translatable strategy for soft tissue augmentation and reconstruction.