The effect of a receptor layer on the measurement of rate constants
The effect of a receptor layer on the measurement of rate constants
复制标题
受体层对速率常数测量的影响
DOI：
10.1006/bulm.2000.0224
复制
发表时间：
2001
影响因子：
3.5
通讯作者：
D. A. Edwards
中科院分区：
文献类型：
--
作者：
D. A. Edwards
许多细胞反应涉及溶液中的反应物与附着在表面的反应物结合或分离。大多数研究假设反应发生在这个表面上，而实际上受体通常位于它上面的薄层上。考虑到这一层的影响，特别是当它与BIAcore™测量设备相关时，尽管结果适用于生物系统。找到了一个测量受体层效应强度的无量纲参数。渐近和奇异摄动技术用于分析缔合和解离动力学，尽管受体层的影响不需要很小。通过分析得到线性和非线性积分方程；对于物理上可实现的情况，构造了显式和渐近解。此外，还推导出了有效速率常数，说明了传输层和受体层对测量速率常数的综合影响。所有这些表达式提供了一个直接的方法来估计速率常数从BIAcore™绑定数据。
Many cellular reactions involve a reactant in solution binding to or dissociating from a reactant attached to a surface. Most studies assume that the reactions occur on this surface, when in actuality the receptors usually lie in a thin layer on top of it. The effect of this layer is considered, particularly as it relates to the BIAcore™ measurement device, though the results are applicable to biological systems. A dimensionless parameter measuring the strength of the effect of the receptor layer is found. Asymptotic and singular perturbation techniques are used to analyse association and dissociation kinetics, though the effect of the receptor layer need not be small. Linear and nonlinear integral equations result from the analysis; explicit and asymptotic solutions are constructed for physically realizable cases. In addition, effective rate constants are derived that illustrate the combined effects of transport and the receptor layer on the measured rate constants. All these expressions provide a direct way to estimate rate constants from BIAcore™ binding data.
影响因子：
3.4
作者：
GOLDSTEIN, B;DEMBO, M
通讯作者：
DEMBO, M
影响因子：
3.4
作者：
Myszka, DG;He, X;Goldstein, B
通讯作者：
Goldstein, B