CHARACTERISTICS OF AN UPTAKE SYSTEM FOR ALPHA-AMINOISOBUTYRIC-ACID IN LEISHMANIA-TROPICA PROMASTIGOTES
CHARACTERISTICS OF AN UPTAKE SYSTEM FOR ALPHA-AMINOISOBUTYRIC-ACID IN LEISHMANIA-TROPICA PROMASTIGOTES
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DOI：
10.1111/j.1550-7408.1983.tb01030.x
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
MUKKADA, AJ
中科院分区：
文献类型：
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作者：
LEPLEY, PR;MUKKADA, AJ
热带乳杆菌前鞭毛体运输α-氨基异丁酸(AIB)，这是中性氨基酸的非代谢类似物，与相当大的浓度梯度相反。AIB不结合到细胞材料中，而是以不变的形式积累在细胞内。细胞内AIB与外部AIB交换。各种能量抑制剂和巯基试剂严重抑制摄取。相对于AIB，摄取系统是饱和的，Lineweaver-Burk图显示了两相动力学，表明参与了两个运输系统。AIB与丙氨酸、半胱氨酸、甘氨酸、蛋氨酸、丝氨酸和脯氨酸具有共同的转运系统。摄取受反馈抑制和反式抑制的调节。
L. tropica promastigotes transport .alpha.-aminoisobutyric acid (AIB), the nonmetabolizable analog of neutral amino acids, against a substantial concentration gradient. AIB is not incorporated into cellular material but accumulates within the cells in an unaltered form. Intracellular AIB exchanges with external AIB. Various energy inhibitors and sulfhydryl reagents severely inhibit uptake. The uptake system is saturable with reference to AIB and the Lineweaver-Burk plots show biphasic kinetics suggesting the involvement of 2 transport systems. AIB shares a common transport system with alanine, cysteine, glycine, methionine, serine and proline. Uptake is regulated by feedback inhibition and transinhibition.