Shaping the zebrafish notochord
Shaping the zebrafish notochord
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DOI：
10.1242/dev.00314
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发表时间：
2003-03-01
期刊：
影响因子：
4.6
通讯作者：
Adams, RJ
中科院分区：
文献类型：
--
作者：
Glickman, NS;Kimmel, CB;Adams, RJ
脊索域在脊椎动物背侧中胚层被指定后，立即经历戏剧性的形态发生。从原肠分裂开始，汇聚和伸展运动将一个蹲着的细胞阵列改变成一个狭窄的、拉长的细胞阵列，它定义了胚胎的主轴。汇聚和延伸可能是由一种高度组织化的细胞混合所连接的，这种混合被称为中间外侧插层行为(MIB)。为了了解MIB是否驱动斑马鱼脊索的早期形态发生，我们进行了4D记录，并定量分析了局部细胞相互作用和背侧中胚层区域形状的整体变化。我们发现，MIB似乎起着调节汇聚的作用，并且可以解释整个背侧中胚层的延伸。
Promptly after the notochord domain is specified in the vertebrate dorsal mesoderm, it undergoes dramatic morphogenesis. Beginning during gastrulation, convergence and extension movements change a squat cellular array into a narrow, elongated one that defines the primary axis of the embryo. Convergence and extension might be coupled by a highly organized cellular intermixing known as mediolateral intercalation behavior (MIB). To learn whether MIB drives early morphogenesis of the zebrafish notochord, we made 4D recordings and quantitatively analyzed both local cellular interactions and global changes in the shape of the dorsal mesodermal field. We show that MIB appears to mediate convergence and can account for extension throughout the dorsal mesoderm.