CELL CYCLING AND DNA-REPLICATION IN A MUTANT BLOCKED IN CELL-DIVISION IN NEMATODE CAENORHABDITIS-ELEGANS
CELL CYCLING AND DNA-REPLICATION IN A MUTANT BLOCKED IN CELL-DIVISION IN NEMATODE CAENORHABDITIS-ELEGANS
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DOI：
10.1016/0012-1606(78)90122-7
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发表时间：
1978-01-01
影响因子：
2.7
通讯作者：
WHITE, JG
中科院分区：
文献类型：
--
作者：
ALBERTSON, DG;SULSTON, JE;WHITE, JG
在单个细胞系的水平上描述了线虫的胚胎后发育。胚胎后发育的突变体lin-5 II导致胚胎后核和细胞分裂失败。在活体动物中，光镜观察到有丝分裂经历了前期和核分裂，但在突变体中形成了一个看起来异常的中期板，在此之后，间期核形态重新出现，直到下一轮分裂。用lin-5研究了腹侧神经索的前体细胞。在野生型中，这些细胞不对称分裂产生6个后代（1个皮下细胞和5个神经元）。在突变体中，这些前体在单个细胞核内积累了大约6倍于二倍体的DNA数量，而有丝分裂则高达3倍。这些多倍体细胞表现出它们通常会产生的细胞的特征。
The postembryonic development of the nematode C. elegans was described at the level of individual cell lineages. A mutant of postembryonic development, lin-5 II, caused a failure of postembryonic nuclear and cell divisions. Mitosis in living animals was seen by light microscopy to proceed through prophase and nuclear breakdown, but an abnormal-looking metaphase plate formed in the mutant, after which the interphase nuclear morphology reappeared until the next attempted round of division. The precursor cells which gave rise to theventral nerve cord were studied in lin-5. In the wild type these cells divided asymmetrically to give 6 descendants (1 hypodermal cell and 5 neurons). In the mutant these precursors accumulated approximately 6 times the diploid quantity of DNA within a single nucleus, while attempting mitosis up to 3 times. These polyploid cells displayed characteristics of the cells they would have produced ordinarily.