RNA N6-methyladenosine modification promotes auxin biosynthesis required for male meiosis in rice
RNA N6-methyladenosine modification promotes auxin biosynthesis required for male meiosis in rice
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DOI：
10.1016/j.devcel.202112.014
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发表时间：
2022-01-24
影响因子：
11.8
通讯作者：
Yu, Hao
中科院分区：
文献类型：
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作者：
Cheng, Peng;Bao, Shengjie;Yu, Hao
N-6-甲基腺苷(m(6)A)的RNA修饰在包括植物在内的生物体中提供了重要的基因调控层；然而，其沉积在参与植物关键发育过程的特定靶标mRNAs上的潜在机制迄今尚不清楚。在这里，我们证明了水稻m(6)A甲基转移酶复合体的核心成分OsFIP37被RNA结合蛋白OsFIP37相关蛋白1(OsFAP1)招募，以在小孢子发生期间介导m(6)A生长素生物合成基因OsYUCCA3的RNA修饰。这稳定了OsYUCCA3mRNA，并促进了雄性减数分裂过程中花药局部生长素的生物合成，这对水稻减数分裂和随后的花粉发育是必不可少的。OsFAP1功能的丧失会导致OsFIP37与OsYUCCA3解离，从而导致m(6)A在OsYUCCA3上的沉积。我们的发现表明，OsFIP37依赖于OsFAP1的m(6)A沉积在OsYUCCA3上，构成了迄今为止未知的RNA修饰与植物生殖发育中雄性减数分裂的激素控制之间的联系。
N-6-methyladenosine (m(6)A) RNA modification confers an essential layer of gene regulation in living organisms, including plants; yet, the underlying mechanisms of its deposition on specific target mRNAs involved in key plant developmental processes are so far unknown. Here, we show that a core component of the rice m(6)A methyltransferase complex, OsFIP37, is recruited by an RNA-binding protein, OsFIP37-associated protein 1 (OsFAP1), to mediate m(6)A RNA modification on an auxin biosynthesis gene, OsYUCCA3, during microsporogenesis. This stabilizes OsYUCCA3 mRNA and promotes local auxin biosynthesis in anthers during male meiosis, which is essential for meiotic division and subsequent pollen development in rice. Loss of function of OsFAP1 causes dissociation of OsFIP37 with OsYUCCA3 and the resulting abolished m(6)A deposition on OsYUCCA3. Our findings reveal that OsFAP1-dependent m(6)A deposition on OsYUCCA3 by OsFIP37 constitutes a hitherto unknown link between RNA modification and hormonal control of male meiosis in plant reproductive development.