Apoptosis inhibitor 5 (API-5; AAC-11; FIF) is upregulated in human carcinomas in vivo
Apoptosis inhibitor 5 (API-5; AAC-11; FIF) is upregulated in human carcinomas in vivo
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DOI：
10.3892/ol.2012.593
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发表时间：
2012-04-01
期刊：
影响因子：
2.9
通讯作者：
Krejci, Pavel
中科院分区：
文献类型：
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作者：
Koci, Lenka;Chlebova, Katarina;Krejci, Pavel
细胞凋亡抑制因子5（apoptosis inhibitor 5，API-5）是一种分子量为55 kDa的核蛋白，在体外肿瘤细胞中具有强的抗细胞凋亡信号转导作用。在这项研究中，我们分析了API-5蛋白在广谱人类癌中的体内表达，包括使用在肿瘤切除过程中获得的肿瘤组织的结肠癌、肺癌、肝癌、肾癌、胰腺癌、胃癌和食道癌。结果显示，与来自邻近正常组织的活检组织相比，肺（23%，n=13）和结肠直肠肿瘤（33%，n=27）的活检组织中API-5表达显著上调。使用结肠癌活组织检查来更密切地研究具有API-5表达水平上调的细胞群。使用基于磁珠的上皮细胞标记EpCAM的选择，我们从肿瘤和对照组织纯化上皮细胞，并通过蛋白质免疫印迹分析这些细胞的API-5表达。我们观察到，与对照EpCAM阳性和从对照或肿瘤组织分离的EpCAM阴性细胞相比，EpCAM阳性肿瘤细胞在所有三个测试的结肠直肠癌病例中表达API-5。这些数据表明，API-5蛋白的表达在肿瘤上皮细胞中上调，并可作为结直肠癌的预后标志物。
Apoptosis inhibitor 5 (API-5) is a 55 kDa nuclear protein with potent anti-apoptotic signaling in tumor cells in vitro. In this study, we analyzed the expression of the API-5 protein in vivo in a broad spectrum of human carcinomas, including those of the colon, lung, liver, kidney, pancreas, stomach and esophagus using tumor tissues obtained during tumor resection. The results showed significant upregulation of API-5 expression in biopsies of lung (23%, n=13) and colorectal tumors (33%, n=27) in comparison with biopsies from the adjacent normal tissue. Colon cancer biopsies were used to study the cell populations with an upregulated level of expression of API-5 more closely. Using a magnetic bead-based selection for the epithelial cell marker EpCAM, we purified epithelial cells from the tumor and control tissues and analyzed these cells for API-5 expression by western immunoblotting. We observed that EpCAM-positive tumor cells expressed API-5 in all three colorectal cancer cases tested, in contrast to the control EpCAM-positive and EpCAM-negative cells isolated from the control or tumor tissues. These data suggest that the expression of the API-5 protein is upregulated in tumor epithelial cells and may serve as a prognostic marker in colorectal cancer.