Synthesis of a Fluorogenic Analogue of Sphingosine-1-Phosphate and Its Use to Determine Sphingosine-1-Phosphate Lyase Activity
Synthesis of a Fluorogenic Analogue of Sphingosine-1-Phosphate and Its Use to Determine Sphingosine-1-Phosphate Lyase Activity
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DOI：
10.1002/cbic.200800809
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发表时间：
2009-03-23
期刊：
影响因子：
3.2
通讯作者：
Fabrias, Gemma
中科院分区：
文献类型：
--
作者：
Bedia, Carmen;Camacho, Luz;Fabrias, Gemma
照亮内质网酶：我们报道了一种荧光化学传感器的设计和合成(1)，用于以高通量筛选格式测量鞘氨醇- 1 -磷酸裂解酶活性，以及使用裂解酶敲除（Sgpl1−/−）细胞对其进行验证。
Illuminating an ER enzyme: We report on the design and synthesis of a fluorogenic chemical sensor (1) to measure sphingosine‐1‐phosphate lyase activity in high‐throughput screening formats, as well as its validation using lyase knockout (Sgpl1−/−) cells.