CRYSTALLIZATION AND TOXICOLOGY OF T34 - A MAJOR TOXIN FROM FLORIDA RED TIDE ORGANISM (PTYCHODISCUS-BREVIS)
CRYSTALLIZATION AND TOXICOLOGY OF T34 - A MAJOR TOXIN FROM FLORIDA RED TIDE ORGANISM (PTYCHODISCUS-BREVIS)
复制标题
DOI：
10.1016/0041-0101(81)90003-9
复制
发表时间：
1981-01-01
期刊：
影响因子：
2.8
通讯作者：
WELLHAM, L
中科院分区：
文献类型：
--
作者：
BADEN, DG;MENDE, TJ;WELLHAM, L
本文报道了从佛罗里达赤潮生物----短假单胞菌的实验室培养物中提纯和结晶一种主要毒素的方法。晶体状毒素溶于丙酮和氯仿，难溶于乙酸乙酯、甲醇和乙醇，微溶于水。晶体毒素在无水分环境中储存时是稳定的。该毒素对鱼类和小鼠具有致死性，在3种体外细胞培养系统中抑制生长，并抑制受精海胆卵的分裂。它对小鼠中针对绵羊红细胞的抗体的形成没有影响，并且不抑制大鼠胶质瘤细胞从成纤维细胞样细胞向胶质样细胞的形态转化。
The purification and crystallization of a major toxin from laboratory cultures of Florida''s red tide organism, P. brevis, was described. The crystalline toxin was soluble in acetone and chloroform, less soluble in ethyl acetate, methanol and ethanol and slightly soluble in water. The crystalline toxin was stable when stored in a moisture-free environment. The toxin was lethal to fish and mice, inhibited growth in 3 in vitro cell culture systems and inhibited the division of fertilized sea urchin eggs. It had no effect on the formation of antibodies directed against sheep red blood cells in mice and did not inhibit the morphological transformation of rat glioma cells from a fibroblast-like to a glial-like cell.