Spartinivicinus marinus sp. nov., isolated from intertidal sediment.
Spartinivicinus marinus sp. nov., isolated from intertidal sediment.
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海米草
DOI：
10.1099/ijsem.0.005919
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发表时间：
2023
影响因子：
2.8
通讯作者：
Xi
中科院分区：
文献类型：
--
作者：
Ji;Xiao;Tie;Jia;Ning;Xiulan Chen;Yu‐Zhong Zhang;Xiao;Xi
从青岛沿海潮间带沉积物中分离到一株革兰氏阴性、需氧、鞭毛状、长杆状细菌，命名为SM 1973 T。菌株SM 1973 T在15-37 °C和0- 5.5%NaCl下生长。 它能将硝酸盐还原为亚硝酸盐，水解秦皮甲素，但不水解酪蛋白和明胶。该菌株16 S rRNA基因序列与大米草（Spartinivicinus ruber）模式菌株的相似性最高（98.2%）。 基于16 S rRNA基因和单拷贝正向聚类的系统发育树显示，菌株SM 1973 T与S.红色，在Zooshikellaceae内形成一个单独的谱系。主要的细胞脂肪酸是特征3（C16：1 ω7с和/或C16：1 ω6с）和C16：0的总和。 主要极性脂质为磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。主要呼吸醌是泛醌-9。菌株SM 1973 T基因组DNA G+C含量为40.4mol%。 根据本文提出的多相证据，菌株SM 1973 T被认为是Spartinivicinus属中的一个新种，并建议将其命名为Spartinivicinus marinus sp. nov.。模式菌株为SM 1973 T（=MCCC 1 K 04833 T =KCTC 72846 T）。
A Gram-stain-negative, aerobic, flagellated, and long rod-shaped bacterium, designated strain SM1973T, was isolated from an intertidal sediment sample collected from the coast of Qingdao, PR China. Strain SM1973T grew at 15-37 °C and with 0-5.5 % NaCl. It reduced nitrate to nitrite and hydrolysed aesculin but did not hydrolyse casein and gelatin. The strain showed the highest 16S rRNA gene sequence similarity (98.2 %) to the type strain of Spartinivicinus ruber. The phylogenetic trees based on the 16S rRNA genes and single-copy orthologous clusters showed that strain SM1973T clustered with S. ruber, forming a separate lineage within the family Zooshikellaceae. The major cellular fatty acids were summed feature 3 (C16 : 1 ω7с and/or C16 : 1 ω6с) and C16 : 0. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The main respiratory quinone was ubiquinone-9. The genomic DNA G+C content of strain SM1973T was 40.4 mol%. Based on the polyphasic evidence presented in this paper, strain SM1973T is considered to represent a novel species within the genus Spartinivicinus, for which the name Spartinivicinus marinus sp. nov. is proposed. The type strain is SM1973T (=MCCC 1K04833T=KCTC 72846T).