Disruption of insulin pathways alters trehalose level and abolishes sexual dimorphism in locomotor activity in Drosophila
Disruption of insulin pathways alters trehalose level and abolishes sexual dimorphism in locomotor activity in Drosophila
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DOI：
10.1002/neu.20193
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发表时间：
2006-01-01
期刊：
影响因子：
--
通讯作者：
Martin, JR
中科院分区：
文献类型：
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作者：
Belgacem, YH;Martin, JR
胰岛素信号通路参与无脊椎动物的多种生理过程，包括生长和寿命的控制；后者也与保幼激素(JH)缺乏有关。反过来，JH水平与不同性别的运动活动差异有关。在这里，胰岛素信号通路参与了果蝇运动活动的性别差异的研究。研究发现，切除成年大鼠大脑间部产生胰岛素的神经元可增加海藻糖血症，并可消除与运动有关的性二型。相反，由胰岛素注射或胰岛素样肽过度表达引起的高胰岛素血症可降低海藻糖血症，但不影响运动行为。此外，我们还发现，在成年果蝇的头部，胰岛素受体(INR)只在大脑周围的脂肪体中表达。虽然男性和女性的INR突变体都是高海藻糖血症，但他们表现出相似的运动活动模式。我们的结果表明，第一，胰岛素控制成年果蝇的海藻糖血症，第二，像JH一样，它以一种独立于其对海藻糖代谢的影响的方式控制成年果蝇运动活动的性别差异。(C)2005年威利期刊公司。
Insulin signaling pathways are implicated in several physiological processes in invertebrates, including the control of growth and life span; the latter of these has also been correlated with juvenile hormone (JH) deficiency. In turn, JH levels have been correlated with sex-specific differences in locomotor activity. Here, the involvement of the insulin signaling pathway in sex-specific differences in locomotor activity was investigated in Drosophila. Ablation of insulin-producing neurons in the adult pars-intercerebralis was found to increase trehalosemia and to abolish sexual dimorphism relevant to locomotion. Conversely, hyper-insulinemia induced by insulin injection or by over-expression of an insulin-like peptide decreases trehalosemia but does not affect locomotive behavior. Moreover, we also show that in the head of adult flies, the insulin receptor (InR) is expressed only in the fat body surrounding the brain. While both male and female InR mutants are hyper-trehalosemic, they exhibit similar patterns of locomotor activity. Our results indicate that first, insulin controls trehalosemia in adults, and second, like JH, it controls sex-specific differences in the locomotor activity of adult Drosophila in a manner independent of its effect on trehalose metabolism. (C) 2005 Wiley Periodicals, Inc.