Extracorporeal shock waves enhance normal fibroblast proliferation in vitro and activate mRNA expression for TGF-β1 and for collagen types I and III
Extracorporeal shock waves enhance normal fibroblast proliferation in vitro and activate mRNA expression for TGF-β1 and for collagen types I and III
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DOI：
10.3109/17453670903316793
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发表时间：
2009-01-01
影响因子：
3.7
通讯作者：
Frairia, Roberto
中科院分区：
文献类型：
--
作者：
Berta, Laura;Fazzari, Annamaria;Frairia, Roberto
背景与目的体外冲击波（ESW）在软组织损伤的治疗中取得了良好的效果，但其作用机制尚不清楚。因此，我们确定了体外ESW对正常成纤维细胞的影响，以评估治疗诱导的细胞反应。（NHDF-12519）用压电装置产生的ESW处理（Piezoson 100; Richard Wolfe）使用不同的脉冲（300、1，000或2，000次发射）和能量（0.11或0.22 mJ/mm（2））协议。在单次冲击波处理后，将未处理的对照和处理的细胞培养12天。在第3、6、9和12天评价细胞活力、生长速率以及TGF β 1和I型和III型胶原mRNA的表达。结果冲击波处理后1 h，细胞活力显示主要与施加的脉冲数相关的下降。用0.22 mJ/mm（2）的能量处理的成纤维细胞随后显示出从第6天到第9天的增殖增加，高于未处理的对照组，而不干扰正常的细胞动力学特征。在第6天和第9天，TGF β-1、第6天I型胶原和第9天III型胶原的mRNA表达在处理的成纤维细胞中也高于未处理的对照组。解释这些体外数据证实，参与结缔组织修复过程的主要因素被ESW激活。该研究给出了ESW治疗肌腱病的基本原理，并可能提供ESW治疗肌腱病的时间表。
Background and purpose Extracorporeal shock waves (ESWs) are used to good effect in the treatment of soft tissue injuries, but the underlying mechanisms are still unknown. We therefore determined the effects of ESWs on normal fibroblasts in vitro, in order to assess treatment-induced cell response.Methods A normal human fibroblast cell line (NHDF-12519) was treated with ESWs generated by a piezoelectric device (Piezoson 100; Richard Wolfe) using different protocols of impulses (300, 1,000, or 2,000 shots) and energy (0.11 or 0.22 mJ/mm(2)). Untreated controls and treated cells were cultivated for 12 days following a single shock-wave treatment. Viability, growth rate, and expression of mRNA for TGF beta-1 and collagen types I and III were evaluated at days 3, 6, 9, and 12.Results 1 hour after shock-wave treatment, cell viability showed a decrease related mainly to impulse numbers applied. Fibroblasts treated with energy of 0.22 mJ/mm(2) subsequently showed an increase in proliferation from day 6 to day 9 that was higher than in untreated controls, without interference with the normal cell kinetic profile. mRNA expression was also higher in treated fibroblasts than in untreated controls for TGF beta-1 on day 6 and day 9, for collagen type I on day 6, and for collagen type III on day 9.Interpretation These in vitro data confirm that the main factors involved in the repair process of connective tissues are activated by ESWs. The study gives the rationale for, and may provide schedules for, ESW treatment of tendonopathies.