Assessment of Altered Cholesterol Homeostasis by Xenobiotics Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry.
Assessment of Altered Cholesterol Homeostasis by Xenobiotics Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry.
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DOI：
10.1002/cptx.65
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发表时间：
2018-11
期刊：
影响因子：
--
通讯作者：
Xu L
中科院分区：
文献类型：
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作者：
Herron J;Hines KM;Xu L
胆固醇和胆固醇衍生的氧固醇对于胚胎发育、突触形成和功能以及髓鞘形成以及其他生物功能至关重要。事实上，胆固醇、固醇前体和氧化固醇水平的改变导致各种发育障碍，强调了胆固醇稳态的重要性。外源性物质通过改变胆固醇稳态来复制相似表型的能力越来越受到关注。因此，定量评估暴露于外源性物质导致的胆固醇稳态改变的能力是有价值的。该单元描述了使用超高效液相色谱-串联质谱法（UHPLC-MS/MS）定量评估各种样品类型中角鲨烯胆固醇生物合成后的改变和随后的氧化甾醇形成的方法。了解胆固醇稳态的改变，导致外源性暴露可以提供关键的洞察毒物的作用机制和由此产生的表型。
Cholesterol and cholesterol-derived oxysterols are critical for embryonic development, synapse formation and function, and myelination, among other biological functions. Indeed, alterations in levels of cholesterol, sterol precursors, and oxysterols results in a variety of developmental disorders, emphasizing the importance of cholesterol homeostasis. The ability of xenobiotics to reproduce similar phenotypes by altering cholesterol homeostasis has increasingly become of interest. Therefore, the ability to quantitatively assess alterations in cholesterol homeostasis resulting from exposure to xenobiotics is of value. This unit describes methods for the quantitative assessment of altered post-squalene cholesterol biosynthesis and subsequent oxysterol formation in various sample types using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Understanding alterations in cholesterol homeostasis resulting from xenobiotic exposure can provide key insight into the toxicant’s mechanism of action and resulting phenotype.