GRA78 encoding a putative S-sulfocysteine synthase is involved in chloroplast development at the early seedling stage of rice
GRA78 encoding a putative S-sulfocysteine synthase is involved in chloroplast development at the early seedling stage of rice
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编码假定的 S-磺基半胱氨酸合酶的 GRA78 参与水稻幼苗期叶绿体的发育
DOI：
10.1016/j.plantsci.2018.12.019
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发表时间：
2018
期刊：
影响因子：
5.2
通讯作者：
Pingrong Wang
中科院分区：
文献类型：
--
作者：
Yang Wang;Ping Zhong;Xiangyu Zhang;Jiqing Liu;Chaoyang Zhang;Xiaorong Yang;Chunmei Wan;Chuanqiang Liu;Hui Zhou;Bin Yang;Changhui Sun;Xiaojian Deng;Pingrong Wang
Cysteine functions not only as an amino acid in proteins but also as a precursor for a large number of essential biomolecules. Cysteine is synthesized via the incorporation of sulfide toO-acetylserine under the catalysis ofO-acetylserine(thiol)lyase (OASTL). In dicotyledonous Arabidopsis, nine OASTL genes have been reported. However, in their null mutants, only the mutant ofCS26encoding S-sulfocysteine synthase showed the visible phenotypic changes, displaying significantly small plants and pale-green leaves under long-day condition but not short-day condition. Up to now, no OASTL gene or mutant has been identified in monocotyledon. In this study, we isolated a green-revertible albino mutantgra78in rice (Oryza sativa). Its albino phenotype at the early seedling stage was sensitive to temperature but independent of photoperiod. Map-based cloning revealed that candidate geneLOC_Os01g59920ofGRA78encodes a putative S-sulfocysteine synthase showing significant similarity with Arabidopsis CS26. Complementation experiment confirmed that mutation inLOC_Os01g59920accounted for the mutant phenotype ofgra78.GRA78is constitutively expressed in all tissues and its encoded protein is targeted to the chloroplast. In addition, qRT-PCR suggested that expression levels of four OASTL homolog genes and five photosynthetic genes were remarkably down-regulated.
