Microarray analyses of gene expression in Japanese flounder Paralichthys olivaceus leucocytes during monogenean parasite Neoheterobothrium hirame infection
Microarray analyses of gene expression in Japanese flounder Paralichthys olivaceus leucocytes during monogenean parasite Neoheterobothrium hirame infection
复制标题
DOI：
10.3354/dao075079
复制
发表时间：
2007-03-29
影响因子：
1.4
通讯作者：
Aoki, Takashi
中科院分区：
文献类型：
--
作者：
Matsuyama, Tomomasa;Fujiwara, Atushi;Aoki, Takashi
为了筛选感染分子生物学标志物，本研究分析了牙鲆感染单殖吸虫过程中外周血白细胞(PBL)的基因表达模式。利用基因芯片技术比较感染鱼和未感染鱼PBL基因的表达模式。在分析的797个基因中，45个基因(5.6%)表达水平发生改变，这些基因包括特异性和非特异性免疫相关基因(基质金属蛋白酶-9、基质金属蛋白酶-13、白三烯B4受体、CD20受体、主要组织相容性复合体I类、MHC II类β链、免疫球蛋白轻链和免疫球蛋白重链)。还观察到一些未知基因的显著上调和下调。选择了几个感染标记基因的候选进行进一步研究。这些基因包括MMP9、MMP13、白三烯B4受体、CD20R、免疫球蛋白重链、免疫球蛋白轻链以及编码为13613、E25、Lb3(8)、We2(3)、We8-18R和Wf12-18R的未知基因。
In this study, the gene expression patterns of peripheral blood leucocytes (PBL) from Japanese flounder Paralichthys olivaceus were analyzed during the course of monogenean parasite Neoheterobothrium hirame infection in order to select candidates for molecular biomarkers of infection. cDNA microarray analysis was performed to compare the gene expression patterns of PBL between infected and non-infected fishes. Among the 797 genes analyzed, 45 genes (5.6 %) changed their expression levels, These genes included specific and non-specific immune-related genes (matrix metalloproteinase[MMP]-9, MMP-13, leukotriene B4 receptor, CD20 receptor, MHC [major histocompatibility complex] Class I, MHC Class II beta-chain, immunoglobulin light chain and immunoglobulin heavy chain). Significant up- and down-regulation of some unknown genes was also observed. Several candidates for infection-marker genes were selected for further study. These genes included MMP-9, MMP-13, leukotriene B4 receptor, CD20 receptor, immunoglobulin heavy chain, immunoglobulin light chain and unknown genes coded as 13613, E25, LB3(8), WE2(3), WE8-18R and WF12-18R.