PET imaging of brain cancer with positron emitter-labeled liposomes
PET imaging of brain cancer with positron emitter-labeled liposomes
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DOI：
10.1016/j.ijpharm.2010.10.001
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发表时间：
2011-01-17
影响因子：
5.8
通讯作者：
Kanazawa, Hiroaki
中科院分区：
文献类型：
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作者：
Oku, Naoto;Yamashita, Mina;Kanazawa, Hiroaki
由于已知脂质体等纳米载体会在荷瘤动物的肿瘤中积聚，而那些捕获了正电子发射器的脂质体可用于PET成像肿瘤，因此我们将（18）f标记的100纳米脂质体用于脑肿瘤成像。聚乙二醇修饰脂质体（已知通过被动靶向在肿瘤中积累）和Ala-Pro-Arg-Pro-Gly修饰的脂质体（已知可进入血管生成部位）被使用。注射后1小时，这些被DiI荧光标记的脂质体在植入大鼠大脑的胶质瘤中积累，但由于血脑屏障的保护，它们不会在正常脑组织中积累。预先形成的脂质体很容易用1-[(18)F]氟-3,6-二氧四环己烷标记，并使PET成像胶质瘤的对比度比用[(18)F]脱氧氟葡萄糖成像的对比度更高。此外，测试中最小的肿瘤，直径为1mm，通过脂质体（18）F成功成像。因此，基于纳米载体的脑肿瘤成像对于脑癌的诊断和可能的基于药物传递的治疗是有希望的。(C) 2010 Elsevier B.V.版权所有
Since nanocarriers such as liposomes are known to accumulate in tumors of tumor-bearing animals, and those that have entrapped a positron emitter can be used to image a tumor by PET, we applied (18)F-labeled 100-nm-sized liposomes for the imaging of brain tumors. Polyethylene glycol (PEG)-modified liposomes, which are known to accumulate in tumors by passive targeting and those modified with Ala-Pro-Arg-Pro-Gly, which are known to home into angiogenic sites were used. Those liposomes labeled with DiI fluorescence accumulated in a glioma implanted in a rat brain 1 h after the injection, although they did not accumulate in the normal brain tissues due to the protection afforded by the blood-brain barrier. Preformed liposomes were easily labeled with 1-[(18)F]fluoro-3,6-dioxatetracosane, and enabled the imaging of gliomas by PET with higher contrast than that obtained with [(18)F]deoxyfluoroglucose. In addition, the smallest tumor among those tested, having a diameter of 1 mm was successfully imaged by the liposomal (18)F. Therefore, nanocarrier-based imaging of brain tumors is promising for the diagnosis of brain cancer and possible drug delivery-based therapy. (C) 2010 Elsevier B.V. All rights reserved.