Novel biomarkers of perchlorate exposure in zebrafish
Novel biomarkers of perchlorate exposure in zebrafish
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DOI：
10.1897/04-270r.1
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发表时间：
2005-05
影响因子：
4.1
通讯作者：
S. Mukhi;J. A. Carr;T. Anderson;R. Patiño
中科院分区：
文献类型：
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作者：
S. Mukhi;J. A. Carr;T. Anderson;R. Patiño
高氯酸盐抑制甲状腺滤泡对碘的摄取，降低甲状腺激素的产生。虽然已经报道了高氯酸盐对甲状腺系统的几种影响，但这些病理学作为环境高氯酸盐暴露的标记的效用还没有得到充分的评估。本研究研究了高氯酸盐对亚成体和成体斑马鱼甲状腺滤泡肥大、胶体耗竭和血管生成的时程和浓度依赖性影响；全身甲状腺激素(T4)水平的变化；以及亚成体和成年斑马鱼的体细胞生长和条件因子。之前在斑马鱼中观察到的胶体T4环强度的变化也被用免疫组织化学方法检测。三个月大的斑马鱼被暴露在高氯酸铵中，高氯酸铵的浓度分别为0，11，90，1,131和11,480 ppb，持续12周，并在干净的水中恢复12周。在暴露2周时，高氯酸盐诱导血管生成和抑制胶体T4环强度的最低观察有效浓度(LOECs)分别为90和1,131 ppb；其他参数未受影响(此时未测定全身T4)。在暴露12周时，胶体耗竭、肥大、血管生成和胶体T4环的LOECs分别为11、480、1、131、90和11 ppb。所有改变都是可逆的，但在恢复12周后，对血管生成和胶体T4环强度的残余影响仍然存在(LOEC，11,480 ppb)。高氯酸盐对全身T4浓度、体长、体重和条件因子均无影响。胶体T4环强度和血管生成变化的敏感性和持久性表明它们作为高氯酸盐暴露的新标记物是有用的。胶体T4环的12周LOEC是水生脊椎动物中报道的高氯酸盐生物标志物中最低的。
Perchlorate inhibits iodide uptake by thyroid follicles and lowers thyroid hormone production. Although several effects of perchlorate on the thyroid system have been reported, the utility of these pathologies as markers of environmental perchlorate exposures has not been adequately assessed. The present study examined time‐course and concentration‐dependent effects of perchlorate on thyroid follicle hypertrophy, colloid depletion, and angiogenesis; alterations in whole‐body thyroxine (T4) levels; and somatic growth and condition factor of subadult and adult zebrafish. Changes in the intensity of the colloidal T4 ring previously observed in zebrafish also were examined immunohistochemically. Three‐month‐old zebrafish were exposed to ammonium perchlorate at measured perchlorate concentrations of 0, 11, 90, 1,131, and 11,480 ppb for 12 weeks and allowed to recover in clean water for 12 weeks. At two weeks of exposure, the lowest‐observed‐effective concentrations (LOECs) of perchlorate that induced angiogenesis and depressed the intensity of colloidal T4 ring were 90 and 1,131 ppb, respectively; other parameters were not affected (whole‐body T4 was not determined at this time). At 12 weeks of exposure, LOECs for colloid depletion, hypertrophy, angiogenesis, and colloidal T4 ring were 11,480, 1,131, 90, and 11 ppb, respectively. All changes were reversible, but residual effects on angiogenesis and colloidal T4 ring intensity were still present after 12 weeks of recovery (LOEC, 11,480 ppb). Whole‐body T4 concentration, body growth (length and weight), and condition factor were not affected by perchlorate. The sensitivity and longevity of changes in colloidal T4 ring intensity and angiogenesis suggest their usefulness as novel markers of perchlorate exposure. The 12‐week LOEC for colloidal T4 ring is the lowest reported for any perchlorate biomarker in aquatic vertebrates.