Genome Wide Host Gene Expression Analysis in Chicken Lungs Infected with Avian Influenza Viruses.
Genome Wide Host Gene Expression Analysis in Chicken Lungs Infected with Avian Influenza Viruses.
复制标题
DOI：
10.1371/journal.pone.0153671
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Raut AA
中科院分区：
文献类型：
--
作者：
Ranaware PB;Mishra A;Vijayakumar P;Gandhale PN;Kumar H;Kulkarni DD;Raut AA
禽流感感染的分子发病机制因禽类个体和病毒株的不同而有很大差异。禽类中高致病性禽流感病毒（HPAIV）或低致病性禽流感病毒（LPAIV）感染的分子发病机制仍知之甚少。因此，使用微阵列研究了感染 HPAI H5N1 (A/duck/India/02CA10/2011) 和 LPAI H9N2 (A/duck/India/249800/2010) 病毒的鸡的整体免疫反应，以确定对这些感染有反应的关键宿主遗传成分。 HPAI H5N1 病毒诱导 I 型 IFN（IFNA 和 IFNG）、细胞因子（IL1B、IL18、IL22、IL13 和 IL12B）、趋化因子（CCL4、CCL19、CCL10 和 CX3CL1）和 IFN 刺激基因（OASL、MX1、RSAD2、IFITM5、IFIT5、GBP 1 和 EIF2AK）在小鼠体内过度表达。肺组织。宿主先天免疫基因的失调可能是鸡流感感染严重程度和结果的关键决定因素。相比之下，大多数这些基因的表达水平在LPAI H9N2病毒感染鸡的肺部中没有被诱导。本研究表明宿主免疫基因与其在鸡 HPAIV 感染发病机制中的作用之间的关系。
The molecular pathogenesis of avian influenza infection varies greatly with individual bird species and virus strain. The molecular pathogenesis of the highly pathogenic avian influenza virus (HPAIV) or the low pathogenic avian influenza virus (LPAIV) infection in avian species remains poorly understood. Thus, global immune response of chickens infected with HPAI H5N1 (A/duck/India/02CA10/2011) and LPAI H9N2 (A/duck/India/249800/2010) viruses was studied using microarray to identify crucial host genetic components responsive to these infection. HPAI H5N1 virus induced excessive expression of type I IFNs (IFNA and IFNG), cytokines (IL1B, IL18, IL22, IL13, and IL12B), chemokines (CCL4, CCL19, CCL10, and CX3CL1) and IFN stimulated genes (OASL, MX1, RSAD2, IFITM5, IFIT5, GBP 1, and EIF2AK) in lung tissues. This dysregulation of host innate immune genes may be the critical determinant of the severity and the outcome of the influenza infection in chickens. In contrast, the expression levels of most of these genes was not induced in the lungs of LPAI H9N2 virus infected chickens. This study indicated the relationship between host immune genes and their roles in pathogenesis of HPAIV infection in chickens.