Palmitoylethanolamide reduces granuloma-induced hyperalgesia by modulation of mast cell activation in rats
Palmitoylethanolamide reduces granuloma-induced hyperalgesia by modulation of mast cell activation in rats
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DOI：
10.1186/1744-8069-7-3
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发表时间：
2011-01-10
期刊：
影响因子：
3.3
通讯作者：
Iuvone, Teresa
中科院分区：
文献类型：
--
作者：
De Filippis, Daniele;Luongo, Livio;Iuvone, Teresa
本研究的目的是获得棕榈酰乙醇胺（PEA）在大鼠慢性肉芽肿性炎症（由肥大细胞（MC）激活持续96小时）中可能的镇痛作用的证据。PEA（200-400-800 μ g/mL），在0时局部给药，与盐水处理的对照组相比，以浓度依赖性方式减少NGF的表达和释放。PEA防止神经形成和发芽，如组织学分析所示，减少机械异常性疼痛，通过Von Frey细丝评价，并抑制背根神经节激活。肉芽肿中MC以脱颗粒为主，紧密分布于神经纤维附近，PEA可显著减少MC脱颗粒和神经纤维形成。这些发现是PEA通过调节MC激活来控制慢性炎症动物模型中的疼痛感知的第一个证据，表明其可能用于治疗所有那些疼痛状况，其中MC激活是最初的关键步骤。
The aim of this study was to obtain evidences of a possible analgesic role for palmitoylethanolamide (PEA) in chronic granulomatous inflammation sustained by mast cell (MC) activation in rats at 96 hours. PEA (200-400-800 mu g/mL), locally administered at time 0, reduced in a concentration-dependent manner the expression and release of NGF in comparison with saline-treated controls. PEA prevented nerve formation and sprouting, as shown by histological analysis, reduced mechanical allodynia, evaluated by Von Frey filaments, and inhibited dorsal root ganglia activation. These results were supported by the evidence that MCs in granuloma were mainly degranulated and closely localized near nerve fibres and PEA significantly reduced MC degranulation and nerves fibre formation. These findings are the first evidence that PEA, by the modulation of MC activation, controls pain perception in an animal model of chronic inflammation, suggesting its potential use for the treatment of all those painful conditions in which MC activation is an initial key step.