Microstructure, bacterial community and metabolic prediction of multi-species biofilms following exposure to di-(2-ethylhexyl) phthalate (DEHP)
Microstructure, bacterial community and metabolic prediction of multi-species biofilms following exposure to di-(2-ethylhexyl) phthalate (DEHP)
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邻苯二甲酸二(2-乙基己基)酯 (DEHP) 暴露后多物种生物膜的微观结构、细菌群落和代谢预测
DOI：
10.1016/j.chemosphere.2019.124382
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发表时间：
2019-12-01
期刊：
影响因子：
8.8
通讯作者：
Li, Wentao
中科院分区：
文献类型：
--
作者：
Li, Yi;Zhang, Peisheng;Li, Wentao
邻苯二甲酸酯在天然和工程来源的生物膜中的发生和运输引起了相当大的研究兴趣。然而，关于邻苯二甲酸酯对多物种生物膜的物理化学结构和细菌群落的影响，目前的研究还很少。邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种模型邻苯二甲酸酯食材，在短期暴露实验（120 h）中，选择它来处理多物种生物膜聚集体，包括来自移动床生物反应器（MBBR）的附着生物膜、来自天然来源的周围植物生物膜和活性污泥。暴露于DEHP后，三种生物膜的胞外聚合物质（EPS）的产量先下降后略有增加，这与腐植酸类有机物组成的最主要荧光化合物的变化一致。在暴露期间，MBBR和周围植物生物膜比活性污泥分泌更多的荧光化合物。EPS中的有机物被转化为更小的分子，而官能团和二级蛋白结构的变化有限。不动杆菌和芽孢杆菌表现出显著的增加，可能是负责DEHP降解的关键属。综合使用光谱、色谱和测序分析表明，周围植物生物膜对DEHP的抵抗力更强，可能是由于存在更成熟的组合，包括具有更高代谢活性的细胞和细菌群落内更高多样性的细胞。本研究揭示了邻苯二甲酸酯暴露后多物种生物膜的微观结构和细菌反应，并为邻苯二甲酸酯的生物修复提供了重要的指导。(C) 2019 Elsevier Ltd.版权所有。
The occurrence and transportation of phthalate esters in biofilms from natural and engineered sources have attracted considerable research interest. However, little information is available highlighting the responses of multi-species biofilms in terms of their physicochemical structure and bacterial community induced by phthalate esters. Di-(2-ethylhexyl) phthalate (DEHP), a model phthalate eater, was selected to treat multi-species biofilm aggregates, including an attached biofilm from a moving bed bioreactor (MBBR), a periphytic biofilm from a natural source and activated sludge in short-term exposure experiments (120 h). The production of extracellular polymeric substances (EPS) from the three biofilms initially decreased and then slightly increased after exposure to DEHP, consistent with the variation of the most dominant fluorescent compounds consisting of humic-acid-like organic substances. The MBBR and periphytic biofilms secreted more fluorescence compounds than the activated sludge during the exposure period. The organic matter in the EPS was converted into smaller molecules, while limited variation was observed in the functional groups and secondary protein structures. Acinetobacter and Bacillus demonstrated significant increases and were likely the key genera responsible for DEHP degradation. The combined use of spectral, chromatographic and sequencing analyses indicated that the periphytic biofilm was more resistant to DEHP, possibly owing to the presence of more mature assemblages, including cells with higher metabolic activity and a higher diversity within the bacterial community. This study provides insights into the microstructural and bacterial responses of multi-species biofilms following exposure to phthalate esters, and provides important guidance for bioremediation of phthalate esters using periphytic biofilms. (C) 2019 Elsevier Ltd. All rights reserved.