Riboswitches in eubacteria sense the second messenger cyclic di-GMP.
Riboswitches in eubacteria sense the second messenger cyclic di-GMP.
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DOI：
10.1126/science.1159519
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发表时间：
2008-07-18
期刊：
影响因子：
--
通讯作者：
Breaker RR
中科院分区：
文献类型：
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作者：
Sudarsan N;Lee ER;Weinberg Z;Moy RH;Kim JN;Link KH;Breaker RR
环状二GMP是一种环状RNA二核苷酸，在不同种类的细菌中作为第二信使发挥作用，引发广泛的生理变化，包括细胞分化、运动和生物膜生活方式之间的转换以及毒力基因表达。然而，环二GMP用于调节基因表达的机制仍然是一个谜。我们证明，在许多细菌物种中的环状二-GMP是通过mRNA中的核糖开关类来感知的，该核糖开关类控制参与许多基本细胞过程的基因的表达。揭示了多种环状二GMP调节子，包括一些与毒力基因表达、皮利形成和鞭毛细胞器生物合成相关的核糖开关。此外，与环状二GMP核糖开关的共有序列匹配的序列存在于噬菌体的基因组中。
Cyclic di-GMP is a circular RNA dinucleotide that functions as a second messenger in diverse species of bacteria to trigger wide-ranging physiological changes, including cell differentiation, conversion between motile and biofilm lifestyles, and virulence gene expression. However, the mechanisms used by cyclic di-GMP to regulate gene expression have remained a mystery. We demonstrate that cyclic di-GMP in many bacterial species is sensed by a riboswitch class in mRNA that controls the expression of genes involved in numerous fundamental cellular processes. A variety of cyclic di-GMP regulons are revealed, including some riboswitches associated with virulence gene expression, pili formation, and flagellar organelle biosynthesis. In addition, sequences matching the consensus for cyclic di-GMP riboswitches are present in the genome of a bacteriophage.