Matrix-assisted laser desorption/ionization imaging mass spectrometry of oxaliplatin derivatives in heated intraoperative chemotherapy (HIPEC)-like treated rat kidney
Matrix-assisted laser desorption/ionization imaging mass spectrometry of oxaliplatin derivatives in heated intraoperative chemotherapy (HIPEC)-like treated rat kidney
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DOI：
10.1002/rcm.4408
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发表时间：
2010-02-01
影响因子：
2
通讯作者：
Larroque, Christian
中科院分区：
文献类型：
--
作者：
Bouslimani, Amina;Bec, Nicole;Larroque, Christian
奥沙利铂[1，2-二氨基环己烷-铂复合体]是一种铂类抗癌药物，主要用于晚期结直肠癌的治疗，特别是术中热化疗(HIPEC)治疗结直肠癌腹膜癌。为了更好地了解奥沙利铂在治疗组织中的渗透情况，我们使用基质辅助激光解吸/电离(MALDI)质谱仪对HIPEC样处理的大鼠肾脏组织切片进行了直接成像。这一程序允许在肾脏切片中检测和定位奥沙利铂及其代谢物，即单半胱氨酸和单蛋氨酸复合体。具体地说，奥沙利铂及其代谢物仅定位于肾皮质，表明它没有深入到器官中。基于这些结果，目前正在对HIPEC后收集的人类肿瘤进行成像分析，以评估奥沙利铂和/或代谢物的分布，目的是确定临床条件，以改善药物渗透。版权所有(C)2010 John Wiley&Sons，Ltd.
Oxaliplatin [1,2-diaminocyclohexane (dach)-Pt complex] is a platinum anticancer drug which is mainly used in the treatment of advanced colorectal cancer, particularly in Heated Intraoperative Chemotherapy (HIPEC) for the treatment of colorectal peritoneal carcinomatosis. In order to better understand the penetration of oxaliplatin in treated tissues we performed a direct imaging of tissue sections from HIPEC-like treated rat kidney using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. This procedure allowed the detection and localization of oxaliplatin and its metabolites, the monocysteine and monomethionine complexes, in kidney sections. Specifically, oxaliplatin and its metabolites were localized exclusively in the kidney cortex, suggesting that it did not penetrate deeply into the organ. Based on these results, an imaging analysis of human tumors collected after HIPEC is currently in progress to assess the distribution of oxaliplatin and/or metabolites with the aim of defining clinical conditions to improve drug penetration. Copyright (C) 2010 John Wiley & Sons, Ltd.