Characterization of messenger RNA expression of estrogen receptor-α and -β in patients with ovarian endometriosis
Characterization of messenger RNA expression of estrogen receptor-α and -β in patients with ovarian endometriosis
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DOI：
10.1016/s0015-0282(00)00527-6
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发表时间：
2000-06-01
影响因子：
6.7
通讯作者：
Yajima, A
中科院分区：
文献类型：
--
作者：
Matsuzaki, S;Fukaya, T;Yajima, A
目的：评价卵巢子宫内膜异位症雌激素受体（ER）-α和ER-β信使RNA（mRNA）的表达。设计：前瞻性研究。设置：大学医院。患者：子宫内膜异位症患者和子宫平滑肌瘤或原位癌患者。干预：在腹腔镜手术中，从32例患者中的每例患者中获取卵巢增生性囊肿组织样本。15例患者在手术过程中或手术后立即采集卵巢组织和子宫内膜样本。主要结果测量：使用逆转录聚合酶链反应（RT-PCR）和非放射性原位杂交（ISH）技术检测ER-α和ER-β mRNA的表达。结果：在RT-PCR和ISH分析中，在来自正常子宫内膜和正常卵巢的所有对照组织中观察到ER-α和ER-β的mRNA表达，尽管在月经周期的不同阶段，ISH分析中阳性信号的分布发生了变化。在所有分析的卵巢囊肿中检测到ER-α的信使RNA（19/19），但在RT-PCR分析中ER-β的mRNA有限（12/19）。结论：ER-α在卵巢子宫内膜异位症的发生、发展过程中起着重要作用，ER-α在上皮细胞和间质细胞中的高表达可能与卵巢子宫内膜异位症的发生、发展有关。((C)2000年由美国生殖医学协会。
Objective: To evaluate the expression of estrogen receptor (ER)-alpha and ER-beta messenger RNA (mRNA) in ovarian endometriosis.Design: Prospective study.Setting: University hospital.Patient(s): Patients with endometriosis and patients with uterine leiomyoma or carcinoma in situ.Intervention(s): A sample of ovarian endometriotic cyst tissue was obtained from each of the 32 patients during laparoscopic cystectomy. Samples of ovarian tissue and endometrium were obtained from 15 patients during or just after surgery.Main Outcome Measure(s): Expression of mRNA for ER-alpha and ER-beta with use of the reverse transcription-polymerase chain reaction (RT-PCR) and nonradioactive in situ hybridization (ISH) techniques.Result(s): Expression of mRNA for ER-alpha and ER-beta was observed in all of the control tissues from the normal endometrium and normal ovaries in the RT-PCR and ISH analyses, although the distribution of positive signals changed in the ISH analysis during different phases of the menstrual cycle. Messenger RNA for ER-alpha was detected in all of the ovarian endometriotic cysts analyzed (19 of 19), but mRNA for the ER-beta was limited (12 of 19) in the RT-PCR analysis. The ISH analysis confirmed the RT-PCR results and revealed that the two estrogen receptors were localized in both epithelial and stromal cells of endometriotic tissues.Conclusion(s): The results suggest that predominant expression of ER-alpha in both glandular epithelial and stromal cells may be essential to the development and growth of ovarian endometriosis. ((C) 2000 by American Society for Reproductive Medicine.)