Quantitation of morphine and codeine in human urine using high-field asymmetric waveform ion mobility spectrometry (FAIMS) with mass spectrometric detection
Quantitation of morphine and codeine in human urine using high-field asymmetric waveform ion mobility spectrometry (FAIMS) with mass spectrometric detection
复制标题
DOI：
10.1093/jat/25.2.81
复制
发表时间：
2001-03-01
影响因子：
2.5
通讯作者：
Guevremont, R
中科院分区：
文献类型：
--
作者：
McCooeye, MA;Ells, B;Guevremont, R
吗啡和可待因已被确定和测量的人尿基质中使用高场不对称波形离子迁移谱（FAIMS）的串联组合与电喷雾电离（ESI）和质谱（MS）检测。在氮气载气中加入氦气，大大提高了电喷雾火焰离子化质谱仪测定吗啡和可待因的灵敏度。人尿液中吗啡和可待因的检测限分别为60 ng/mL和20 ng/mL，分析前无需对样品进行净化、衍生化或色谱分离。
Morphine and codeine have been identified and measured in a human urine matrix using high-field asymmetric waveform ion mobility spectrometry (FAIMS) in a tandem combination with electrospray ionization (ESI) and mass spectrometric (MS) detection. The addition of helium to the nitrogen carrier gas resulted in a substantial improvement in the sensitivity of the ESI-FAIMS-MS instrument for the determination of morphine and codeine. Limits of detection in human urine were 60 ng/mL for morphine and 20 ng/mL for codeine with no clean-up, derivatization, or chromatographic separation of the sample prior to analysis.