Detection of amyloid-beta by Fmoc-KLVFF self-assembled fluorescent nanoparticles for Alzheimer's disease diagnosis
Detection of amyloid-beta by Fmoc-KLVFF self-assembled fluorescent nanoparticles for Alzheimer's disease diagnosis
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Fmoc-KLVFF 自组装荧光纳米颗粒检测淀粉样蛋白用于阿尔茨海默病诊断
DOI：
10.1016/j.cclet.2020.09.009
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发表时间：
2021-04-21
影响因子：
9.1
通讯作者：
Sun, Leming
中科院分区：
文献类型：
--
作者：
Liu, Dingchang;Fu, Dongjie;Sun, Leming
淀粉样蛋白β（A/3）的异常聚集被广泛认为在阿尔茨海默病（AD）的发病机制中起重要作用，也被认为是AD诊断的主要生物标志物之一。肽序列Lys-Leu-Val-Phe-Phe（KLVFF）被认为是A/3原纤化的主要驱动因素，其也可用于靶向A/3并抑制其聚集。在本研究中，KLVFF和Fmoc-KLVFF荧光纳米粒子通过锌配位和p-p堆积自组装。荧光纳米颗粒对A/3聚集体（包括寡聚体和原纤维）的识别可以通过芳香、疏水和氢键相互作用来实现。荧光纳米探针可以区分A/3聚集形式，并在1 pg/mL（S/N = 3）的限度下检测A/3。因此，通过荧光肽纳米颗粒检测A/3聚集体对于AD诊断和进展预测具有巨大的潜力。(C)2020中国化学会、中国医学科学院药物研究所。Elsevier B. V.出版，保留所有权利。
The abnormal aggregation of amyloid-beta (A/3) has been widely believed to play an important role in the pathogenesis of Alzheimer's disease (AD), which is also recognized as one of the main biomarkers for AD diagnosis. The peptide sequence Lys-Leu-Val-Phe-Phe (KLVFF) is considered as the main driver of the fibrillation of A/3, which also can be utilized to target A/3 and inhibit its aggregation. In this study, KLVFF and Fmoc-KLVFF fluorescent nanoparticles were self-assembled through zinc coordination and p -p stacking. The recognition of A/3 aggregates including oligomers and fibrils by fluorescent nanoparticles can be realized through aromatic, hydrophobic, and hydrogen-bond interactions. The fluorescent nanoprobes can distinguish A/3 aggregation formats and detect A/3 at the limit of 1 pg/mL (S/N = 3). Hence, the detection of A/3 aggregates by fluorescent peptide nanoparticles has great potential for AD diagnosis and progression prediction. (C) 2020 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.