Optical saturation in fluorescence correlation spectroscopy under continuous-wave and pulsed excitation
Optical saturation in fluorescence correlation spectroscopy under continuous-wave and pulsed excitation
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DOI：
10.1002/cphc.200400319
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发表时间：
2005-01-01
期刊：
影响因子：
2.9
通讯作者：
Enderlein, J
中科院分区：
文献类型：
--
作者：
Gregor, I;Patra, D;Enderlein, J
对光饱和效应引起的荧光相关测量与光激发功率的关系进行了详细的理论和实验研究。结果表明，随着激发功率的降低，荧光相关测量对激发功率的灵敏度越来越高。这使得用荧光相关光谱准确测量扩散系数变得相当困难。对于连续波激励和脉冲激励，这一行为有很大的不同。
A detailed theoretical and experimental study of the dependence of fluorescence correlation measurements on optical excitation power due to optical saturation effects is presented. It is shown that the sensitivity of a fluorescence correlation measurement on excitation power becomes increasingly stronger for decreasing excitation power. This makes exact measurements of diffusion coefficients with fluorescence correlation spectroscopy rather difficult. A strong difference of this behavior for continuous-wave and pulsed excitation is found.