Evaluation of a novel Wave Bioreactor® cellbag for aerobic yeast cultivation
Evaluation of a novel Wave Bioreactor® cellbag for aerobic yeast cultivation
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DOI：
10.1007/s00449-007-0119-y
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发表时间：
2007-07-01
影响因子：
3.8
通讯作者：
Amanullah, Ashraf
中科院分区：
文献类型：
--
作者：
Mikola, Mark;Seto, Jennifer;Amanullah, Ashraf
由于一次性技术和易于使用的优点，Wave Bioreator(R)在细胞培养中被广泛使用。一种新型的手提袋被开发出来，它带有一个弗里特喷雾器，以增加系统的氧气转移。本工作的目的是对用于酵母培养的泡沫袋进行评价。在模拟培养基中进行了氧气传质研究，得出该体系的最大氧气传质系数(k(L)a)为38h(-1)。这些测量表明，Sparger在增加氧传递能力方面没有效果。酿酒酵母在充氧、充气和充氧标准纤维袋中培养成功。在稳态条件下，两种细胞袋设计的k(L)a值高达60h(-1)，生长特性没有差异。这是在波浪生物反应器(R)中成功培养微生物的第一个报告，比较了摇瓶和搅拌槽式生物反应器中的常规种子膨胀。
The Wave Bioreactor (R) is widely used in cell culture due to the benefits of disposable technology and ease of use. A novel cellbag was developed featuring a frit sparger to increase the system's oxygen transfer. The purpose of this work was to evaluate the sparged cellbag for yeast cultivation. Oxygen mass transfer studies were conducted in simulated culture medium and the sparged system's maximum oxygen mass transfer coefficient (k(L) a) was 38 h(-1). These measurements revealed that the sparger was ineffective in increasing the oxygen transfer capacity. Cultures of Saccharomyces cerevisiae were successfully grown in oxygen-blended sparged and oxygen-blended standard cellbags. Under steady state conditions for both cellbag designs, k(L) a values as high as 60 h(-1) were obtained with no difference in growth characteristics. This is the first report of a successful cultivation of a microbe in a Wave Bioreactor (R) comparing conventional seed expansion in shake flasks and stirred tank bioreactors.