Reduction of Cedar Pollen Adhesion by Lecithin Polymer Coating
Reduction of Cedar Pollen Adhesion by Lecithin Polymer Coating
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通过卵磷脂聚合物涂层减少雪松花粉的粘附
DOI：
10.2332/allergolint.54.573
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发表时间：
2005
影响因子：
6.8
通讯作者：
Y. Kohno
中科院分区：
文献类型：
--
作者：
Yusuke Kobayashi;K. Sakuma;J. Hosoi;Yuki Suda;Katsuyuki Maeno;K. Miyazawa;H. Ichikawa;Y. Kohno
摘要背景避免过敏原是包括花粉症在内的过敏性疾病最有效的治疗方法之一。虽然市场上有各种花粉症患者用的口罩和护目镜，但在防止花粉粘附到人体和衣服上方面做的工作很少。我们已经研究了聚合物涂层对雪松花粉粘附到皮肤、头发和织物上的影响，并发现含有阳离子和阴离子位点的卵磷脂聚合物（LP）在降低粘附水平方面非常有效。方法我们评估了样品表面涂覆阴离子聚合物（AP）、阳离子聚合物（CP）、非离子聚合物（NIP）或LP（每种都通过喷涂0.10%聚合物的乙醇/水溶液）后静电荷和摩擦系数的变化。花粉粘附水平进行了评估，通过观察的数量雪松花粉颗粒的视频显微镜（VMS）图像和雪松花粉抗原Cry j1的定量与夹心ELISA系统。用VMS观察花粉对人皮肤或头发的粘附。结果涂脂后试样的静电荷水平和摩擦系数均显著降低。我们推测，这些属性是密切相关的雪松花粉或哭j1的粘附水平相比，CP，AP或NIP涂层样品的显着减少。在经LP处理的皮肤和经LP处理的上蜡毛发上观察到类似的效果。结论我们的研究结果表明，LP涂层可以通过减少患者与雪松花粉抗原的接触水平来帮助花粉症的管理。
ABSTRACT Background Avoidance of allergens is one of the most effective treatments of allergenic diseases, including pollinosis. Although various masks and goggles for pollinosis sufferers are commercially available, little work has been done on preventing adhesion of pollen to the human body and clothes. We have examined the effect of polymer coating on adhesion of cedar pollen to skin, hair and fabrics, and found that a lecithin polymer (LP), containing both cationic and anionic sites, is highly effective in reducing adhesion levels. Methods We evaluated the changes in electrostatic charge and frictional coefficient of sample surfaces after coating with an anionic polymer (AP), a cationic polymer (CP), a nonionic polymer (NIP), or LP (each applied by spraying 0.10% polymer solution in ethanol/water). Pollen adhesion level was evaluated by observation of the number of cedar pollen particles on video microscope (VMS) images and by quantification of cedar pollen antigen Cry j1 with a sandwich ELISA system. Adhesion of pollen to human skin or hair was also examined by VMS observation. Results LP-coated samples showed a significant reduction in electrostatic charge level and frictional coefficient. We presumed that these properties are closely related to the significant reduction in the adhesion level of cedar pollen or Cry j1 compared with CP, AP or NIP-coated samples. A similar effect was seen on LP-treated skin, and LP-treated waxed hair. Conclusions Our results suggest that coating with LP could help in the management of pollinosis by reducing the contact level of patients with cedar pollen antigens.