Up-regulation of defense enzymes is responsible for low reactive oxygen species in malignant prostate cancer cells
Up-regulation of defense enzymes is responsible for low reactive oxygen species in malignant prostate cancer cells
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DOI：
10.1038/emm.2005.62
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发表时间：
2005-10-31
影响因子：
12.8
通讯作者：
Lim, CJ
中科院分区：
文献类型：
--
作者：
Lim, HW;Hong, S;Lim, CJ
活性氧（Reactive oxygen species，ROS）参与了肿瘤发生发展过程中的许多重要现象。为了研究氧化应激及其相关系统在肿瘤进展中的变化，在前列腺癌细胞系PC 3和LNCaP中检测了抗氧化应激防御系统中的多种组分。与LNCaP细胞相比，参与转移的细胞表面分子在PC 3细胞中高表达，并且仅在PC 3细胞中显示出强的侵袭能力。LNCaP细胞中的ROS水平是PC 3细胞中的两倍，尽管两种细胞系之间的一氧化氮（NO）水平相似。与LNCaP相比，PC 3中GSH的含量增加了约2倍。谷胱甘肽还原酶，硫氧还蛋白还原酶和谷胱甘肽S-转移酶的活性，除了过氧化氢酶是显着高于在PC 3细胞比LNCaP细胞。此外，氧化应激诱导剂引起的GSH和谷胱甘肽S-转移酶的下调LNCaP细胞比PC 3细胞更显着。这些结果意味着，恶性肿瘤细胞可以保持较低的活性氧含量保持相对较高的抗氧化能力，即使在应激剂的存在下。
Reactive oxygen species (ROS) are involved in a diversity of important phenomena in the process of tumor development. To investigate the alterations of oxidative stress and their related systems in tumor progression, a variety of components in the antioxidative stress defense system were examined in prostate cancer cell lines, PC3 and LNCaP. Cell surface molecules involved in metastasis were expressed highly in PC3 cells compared with LNCaP cells, and strong invasion ability was shown in PC3 cells only. ROS level in LNCaP cells was twice higher than that in PC3 cells, although nitric oxide (NO) level was similar between the two cell lines. The content of GSH increased up to about 2-fold in PC3 compared with LNCaP. Activities of glutathione reductase, thioredoxin reductase, and glutathione S-transferase except catalase are significantly higher in PC3 cells than in LNCaP cells. Furthermore,, oxidative stress-inducing agents caused down-regulation of GSH and glutathione S-transferase much more significantly in LNCaP cells than in PC3 cells. These results imply that malignant tumor cells may maintain low ROS content by preserving relatively high anti-oxidative capacity, even in the presence of stressful agents.