Small Circular DNA Molecules Act as Rigid Motifs To Build DNA Nanotubes
Small Circular DNA Molecules Act as Rigid Motifs To Build DNA Nanotubes
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DOI：
10.1021/ja504050r
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发表时间：
2014-07-23
影响因子：
15
通讯作者：
Xiao, Shou-Jun
中科院分区：
文献类型：
--
作者：
Zheng, Hongning;Xiao, Minyu;Xiao, Shou-Jun
具有设计长度的小环状DNA分子，例如64和96个核苷酸（nt），在分别与几个32-nt的钉合链杂交后，可以充当用于构建具有优异的环直径均匀性的DNA纳米管的刚性基序。与大多数由纵向双螺旋组成的天然DNA纳米管不同，由圆形DNA组装的纳米管由横向双螺旋构成。在这里设计的五种类型的DNA纳米管中，四种是通过交替两个相同环大小的不同环构建的，而一种是由所有相同的96-nt环组成的。由相同的96-nt环构建的纳米管比由两个不同的96-nt环构建的纳米管短10-100倍，因为环上的铰链接头较少。
Small circular DNA molecules with designed lengths, for example 64 and 96 nucleotides (nt), after hybridization with a few 32-nt staple strands respectively, can act as rigid motifs for the construction of DNA nanotubes with excellent uniformity in ring diameter. Unlike most native DNA nanotubes, which consist of longitudinal double helices, nanotubes assembled from circular DNAs are constructed from lateral double helices. Of the five types of DNA nanotubes designed here, four are built by alternating two different rings of the same ring size, while one is composed of all the same 96-nt rings. Nanotubes constructed from the same 96-nt rings are 10-100 times shorter than those constructed from two different 96-nt rings, because there are fewer hinge joints on the rings.