Simple and rapid chemiluminescence aptasensor for Hg2+ in contaminated samples: A new signal amplification mechanism
Simple and rapid chemiluminescence aptasensor for Hg2+ in contaminated samples: A new signal amplification mechanism
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简单快速的化学发光适体传感器检测污染样品中的 Hg2：一种新的信号放大机制。
DOI：
10.1016/j.bios.2016.08.022
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发表时间：
2017-01-15
影响因子：
12.6
通讯作者：
Li, Baoxin
中科院分区：
文献类型：
--
作者：
Qi, Yingying;Xiu, Fu-Rong;Li, Baoxin
超低浓度重金属Hg 2+在环境和生物领域中具有逐渐积累的特性，其检测对人类健康保护和环境监测具有重要意义。本文报道了一种基于正电荷金纳米颗粒（（+）AuNPs）信号放大机制的化学发光（CL）生物传感平台。它是基于（+）AuNPs的电荷效应和靶点诱导的适体构象变化，在H2 O2和鲁米诺存在下，无高盐介质条件下刺激CL的产生。特别值得注意的是，来自（-）AuNPs系统的高盐介质的典型问题，如影响适体与靶标的结合和阻碍CL反应，可以通过直接引入（+）AuNPs来有效地解决。因此，所提出的生物传感对目标Hg 2+表现出高灵敏度，检测限为16 pM，远低于美国环境保护署在饮用水中定义的限度（10 nM），并且比先前报道的基于适体的Hg 2+测定低约10倍。该传感平台为汞离子的无标记灵敏检测提供了一种简单、快速且具有成本效益的方法。此外，它对于其他目标的检测是通用的。这种直接利用（+）AuNPs电荷效应的方法无疑为无标记核酸适体化学发光分析提供了一种新的信号放大途径。(C)2016爱思唯尔B. V.保留所有权利。
Detection of ultralow concentration of heavy metal ion Hg2+ is important for human health protection and environment monitoring because of the gradual accumulation in environmental and biological fields. Herein, we report a convenient chemiluminescence (CL) biosensing platform for ultrasensitive Hg2+ detection by signal amplification mechanism from positively charged gold nanoparticles ((+)AuNPs). It is based on (+)AuNPs charge effect and aptamer conformation change induced by target to stimulate the generation of CL in the presence of H2O2 and luminol without high salt medium. Notably particularly, the typical problem of the high salt medium from (-) AuNPs system, like influencing aptamers' bind with target and hindering CL reaction can be effectively addressed through the direct introduction of (+) AuNPs. Therefore, the proposed biosensing exhibits a high sensitivity toward target Hg2+ with a detection limit of 16 pM, which is far below the limit (10 nM) defined by the U.S. Environmental Protection Agency in drinkable water, and is about 10-fold lower than the previously reported aptamer-based assays for Hg2+. This sensing platform provides a simple, rapid, and cost-effective approach for label-free sensitive detection of Hg2+. Moreover, it is universal for the detection of other targets. Undoubtedly, such a direct utilizing of (+)AuNPs' charge effect will provide a new signal amplification way for label-free aptamer-based CL analysis. (C) 2016 Elsevier B.V. All rights reserved.