Plasma miRNA expression profiles in rheumatoid arthritis associated interstitial lung disease.
Plasma miRNA expression profiles in rheumatoid arthritis associated interstitial lung disease.
复制标题
类风湿性关节炎相关间质性肺疾病中的血浆 miRNA 表达谱。
DOI：
10.1186/s12891-017-1389-4
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发表时间：
2017-01-19
影响因子：
2.3
通讯作者：
Tohma S
中科院分区：
文献类型：
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作者：
Oka S;Furukawa H;Shimada K;Hashimoto A;Komiya A;Fukui N;Tsuchiya N;Tohma S
间质性肺病（ILD）常与类风湿性关节炎（RA）相关，称为RA相关ILD（RA-ILD）。RA-ILD对RA的预后有很大影响。在这里，我们研究了microRNAs（miRNAs）谱，以确定它们是否可用于诊断RA-ILD。从血浆样品中分离RNA并合成cDNA。进行实时RT-PCR分析，以评估来自RA伴或不伴RA-ILD患者的血浆池中的752个miRNA表达谱。在来自64名患有或不患有RA-ILD的RA患者的个体血浆中分析了16种选定的miRNA水平。hsa-miR-214- 5 p的表达水平（平均相对表达水平±标准差，RA伴ILD组为8.1 ± 28.2，RA不伴ILD组为0.2 ± 0.9，P = 0.0156）和hsa-miR-7- 5 p RA伴ILD组56.2 ± 260.4，RA不伴ILD组4.7 ± 11.8，P = 0.0362。RA合并ILD患者的hsa-miR-214- 5 p和hsa-miR-7- 5 p产生的ILD的miRNA指数（214，7）值显著高于RA未合并ILD患者（0.122 ± 0.332，0.006 ± 0.013，P = 0.0010）。miRNA指数（214，7）的受试者工作特征曲线的曲线下面积为0.740。据我们所知，这是第一份关于RA-ILD中miRNA谱的报告。hsa-miR-214- 5 p和hsa-miR-7- 5 p在RA合并ILD患者中的表达水平升高。本文的在线版本（doi：10.1186/s12891-017-1389-4）包含补充材料，可供授权用户使用。
Interstitial lung disease (ILD) is frequently associated with rheumatoid arthritis (RA), and is designated RA-associated ILD (RA-ILD). RA-ILD has a large impact on the prognosis of RA. Here, we investigated the micro RNAs (miRNAs) profiles to determine whether they may be useful for diagnosing RA-ILD. RNA was isolated from plasma samples and cDNA was synthesized. Real-time RT-PCR analysis was performed to evaluate 752 miRNA expression profiles in plasma pools from RA patients with or without RA-ILD. Sixteen selected miRNA levels were analyzed in individual plasmas from 64 RA patients with or without RA-ILD. Expression levels of hsa-miR-214-5p (mean relative expression level ± standard deviation, 8.1 ± 28.2 in RA with ILD, 0.2 ± 0.9 in RA without ILD, P = 0.0156) and hsa-miR-7-5p (56.2 ± 260.4 in RA with ILD, 4.7 ± 11.8 in RA without ILD, P = 0.0362) were higher in RA patients with RA-ILD than in those without. The values of miRNA index (214, 7) generated from hsa-miR-214-5p and hsa-miR-7-5p for ILD were significantly elevated in RA patients with RA-ILD compared with those without (0.122 ± 0.332 in RA with ILD, 0.006 ± 0.013 in RA without ILD, P = 0.0010). The area under the curve value of the receiver operating characteristic curve for the miRNA index (214, 7) was 0.740. To the best of our knowledge, this is the first report of miRNA profiles in RA-ILD. The expression levels of hsa-miR-214-5p and hsa-miR-7-5p were increased in RA with ILD. The online version of this article (doi:10.1186/s12891-017-1389-4) contains supplementary material, which is available to authorized users.