VALIDITY OF MITOCHONDRIAL MARKER ENZYMES IN RAT HEART
VALIDITY OF MITOCHONDRIAL MARKER ENZYMES IN RAT HEART
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DOI：
10.1016/0022-2828(71)90043-5
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发表时间：
1971-01-01
影响因子：
5
通讯作者：
NAHORSKI, SR
中科院分区：
文献类型：
--
作者：
PYCOCK, CJ;NAHORSKI, SR
几种酶，以前已被用来确定完整的线粒体的活动已被检查和严格评估从大鼠心脏homogenates.The本地化的酶（琥珀酸脱氢酶，脱氢酶，异柠檬酸脱氢酶（NADP-和NAD-依赖），NADH氧化酶和单胺氧化酶）的亚细胞组分已被比较直接从电子显微镜研究中获得的视觉证据。此外，还对线粒体组织化学染色Janus绿色B的定量测定进行了相关性分析，得出结论：琥珀酸脱氢酶和异柠檬酸脱氢酶（NAD依赖性）是大鼠心肌线粒体最理想的指示酶。
The activities of several enzymes that have previously been utilized to identify intact mitochondria have been examined and critically evaluated in subcellular fractions obtained from rat cardiac homogenates.The localization of the enzymes investigated (succinate dehydrogenase, fumarase, isocitrate dehydrogenase (NADP- and NAD-dependent), NADH oxidase and monoamine oxidase) have been compared directly with the visual evidence obtained from electron microscopy studies. In addition the quantitative estimation of the mitochondrial histochemical stain Janus Green B was correlated with these studies.It was concluded that the most satisfactory mitochondrial indicator enzymes in rat heart are succinate dehydrogenase and isocitrate dehydrogenase (NAD-dependent).