Gene-sized macronuclear DNA molecules are clustered in micronuclear chromosomes of the ciliate Oxytricha nova.
Gene-sized macronuclear DNA molecules are clustered in micronuclear chromosomes of the ciliate Oxytricha nova.
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基因大小的大核 DNA 分子聚集在纤毛虫尖毛虫的微核染色体中。
DOI：
10.1128/mcb.6.11.3606-3613.1986
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发表时间：
1986
影响因子：
5.3
通讯作者：
Ribas-Aparicio,RM
中科院分区：
文献类型：
--
作者：
Klobutcher,LA;Vailonis-Walsh,AM;Cahill,K;Ribas-Aparicio,RM
在其生活史的有性阶段之后，Hypotrichous ciliateOxytricha novatransforms将其染色体微核的拷贝转化为含有平均大小为2.2个DNA酶对的短线性DNA分子的大核。此外，在这个过程中，超过90%的DNA序列在微核基因组中被消除。我们已经研究了大核DNA分子在微核染色体中的组织。在两个克隆的微核DNA片段中，发现大核DNA分子聚集在一起，并且相隔不到550个碱基对。还分离了微核中相邻的两个大核DNA分子的重组克隆，并通过DNA测序进行了检查。发现这两个大核DNA分子在微核基因组中仅相隔90个碱基对。
Following the sexual phase of its life cycle, the hypotrichous ciliateOxytricha novatransforms a copy of its chromosomal micronucleus into a macronucleus containing short, linear DNA molecules with an average size of 2.2 kilobase pairs. In addition, more than 90% of the DNA sequences in the micronuclear genome are eliminated during this process. We have examined the organization of macronuclear DNA molecules in the micronuclear chromosomes. Macronuclear DNA molecules were found to be clustered and separated by less than 550 base pairs in two cloned segments of micronuclear DNA. Recombinant clones of two macronuclear DNA molecules that are adjacent in the micronucleus were also isolated and examined by DNA sequencing. The two macronuclear DNA molecules were found to be separated by only 90 base pairs in the micronuclear genome.