Changes in soil microbial community functionality and structure in a metal-polluted site: The effect of digestate and fly ash applications
Changes in soil microbial community functionality and structure in a metal-polluted site: The effect of digestate and fly ash applications
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DOI：
10.1016/j.jenvman.2015.07.042
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发表时间：
2015-10-01
影响因子：
8.7
通讯作者：
Szakova, J.
中科院分区：
文献类型：
--
作者：
Garcia-Sanchez, M.;Garcia-Romera, I.;Szakova, J.
Trhove Dusniky（捷克共和国普里布拉姆）土壤的特点是，由于采矿和冶炼活动，其铅、银、锌多金属积累量很高。在我们的研究小组以前进行的研究中，我们已经评估了可能使用的修正，这将减少汞等金属的流动性和可用性。我们已经观察到，在金属污染的土壤中施用硫酸盐和粉煤灰对固定这些金属有影响。然而，到目前为止，我们还缺乏有关这些修正案对土壤微生物功能和社区的影响的信息。采用盆栽试验研究了复合污染土壤中小麦生长过程中不同处理对土壤微生物群落的影响。处理30天和60天后采集土壤样品。由于碳酸盐态中C和N的含量较高，碳酸盐态的施用改善了化学属性，如总有机碳（TOC）、水溶性碳（WSOC）、总可溶性碳（C）、总可溶性氮（N）和无机氮形态（NO3-）的含量。同样，微生物的活性大大提高了由亚硝酸盐的应用，因为是生理多样性。细菌和真菌群落增加，微生物生物量大大提高。这些效果在治疗30天和60天后明显。相比之下，粉煤灰没有显着的效果相比，秸秆状态，但土壤微生物生物量的积极影响，作为一个结果的宏观和微观营养源的应用，通过添加粉煤灰。本研究表明，亚硝酸盐可以成功地应用于重金属污染土壤的修复。(C)2015爱思唯尔有限公司版权所有。
Soil from Trhove Dusniky (Pribram, Czech Republic) is characterized by its high polymetallic accumulations in Pb-Ag-Zn due to mining and smelting activities. In previous studies performed in our research group, we have evaluated the potential use of amendments that would reduce the mobility and availability of metals such as Hg. We have observed that the application of digestate and fly ash in metal-polluted soil has an impact in immobilizing these metals. However, until now we have lacked information about the effect of these amendments on soil microbial functionality and communities. The multi-contaminated soil was used to grow wheat in a pot experiment to evaluate the impact of digestate and fly ash application in soil microbial communities. Soil samples were collected after 30 and 60 days of treatment. The digestate application improved chemical attributes such as the content in total organic carbon (TOC), water soluble carbon (WSOC), total soluble carbon (C), total soluble nitrogen (N), and inorganic N forms (NO3-) as consequence of high content in C and N which is contained in digestate. Likewise, microbial activity was greatly enhanced by digestate application, as was physiological diversity. Bacterial and fungal communities were increased, and the microbial biomass was highly enhanced. These effects were evident after 30 and 60 days of treatment. In contrast, fly ash did not have a remarkable effect when compared to digestate, but soil microbial biomass was positively affected as a consequence of macro- and micro-nutrient sources applied by the addition of fly ash. This study indicates that digestate can be used successfully in the remediation of metal-contaminated soil. (C) 2015 Elsevier Ltd. All rights reserved.