Neutralizing antibodies after infection with dengue 1 virus.
Neutralizing antibodies after infection with dengue 1 virus.
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DOI：
10.3201/eid1302.060539
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发表时间：
2007-02
影响因子：
11.8
通讯作者：
Halstead SB
中科院分区：
文献类型：
--
作者：
Guzman MG;Alvarez M;Rodriguez-Roche R;Bernardo L;Montes T;Vazquez S;Morier L;Alvarez A;Gould EA;Kouri G;Halstead SB
当感染2型登革病毒后间隔20年感染2型登革病毒时，疾病的严重程度显著增加。当在感染DENV-1之后感染2型登革病毒（DENV-2）时，疾病的严重程度显著增加。研究表明，异源中和抗体滴度与第二次感染的严重程度呈负相关。如果这种机制控制了古巴的疾病严重程度，异型抗体滴度应该随着时间的推移而下降。为了确定登革热抗体的表型是否随时间发生变化，我们分析了DENV-1感染后4-8年和20-22年收集的血清样本。我们发现同源DENV-1中和抗体的平均滴度显著增加，而针对DENV-2病毒的2种基因型之一（美国基因型）的异源抗体显著减少。亚洲DENV-2病毒在任一时间间隔内均未被中和;然而，美国基因型在异型病毒中和抗体中沿预测方向发生表型变化。这一发现可能与继发性登革热感染的疾病严重程度的时间依赖性变化有关。
Severity of disease is markedly increased when infection with dengue virus type 2 follows infection with dengue virus type 2 by an interval of 20 years. Severity of disease is markedly increased when infection with dengue virus type 2 (DENV-2) follows infection with DENV-1. Studies have shown that heterologous neutralizing antibody titers are inversely correlated with severity of a second infection. If this mechanism controlled disease severity in Cuba, heterotypic antibody titers should have declined over time. To determine whether phenotypic changes in dengue antibodies occur over time, we analyzed serum samples collected 4–8 and 20–22 years after DENV-1 infection. We found a significant increase in mean titer of homologous DENV-1 neutralizing antibodies and a significant decrease in heterologous antibodies to 1 of 2 genotypes of DENV-2 virus (the American genotype). Asian DENV-2 viruses were not neutralized during either interval; however, the American genotype underwent phenotypic changes in heterotypic viral neutralizing antibodies in the predicted direction. This finding may be related to the time-dependent changes in severity of disease found with secondary dengue infection.