The Mode of Action of Endosidin20 Differs from That of Other Cellulose Biosynthesis Inhibitors
The Mode of Action of Endosidin20 Differs from That of Other Cellulose Biosynthesis Inhibitors
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DOI：
10.1093/pcp/pcaa136
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发表时间：
2020-12-01
影响因子：
4.9
通讯作者：
Zhang, Chunhua
中科院分区：
文献类型：
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作者：
Huang, Lei;Zhang, Chunhua
内皮素20（Endosidin20，ES20）是近年来发现的一种纤维素生物合成抑制剂（CELLULOSE biosynthesis inhibitor，CBI），其作用靶点是纤维素合成酶6（CESA 6）的催化结构域，从而抑制拟南芥的生长。在这里，我们表征了ES 20对其他植物物种生长的影响，发现ES 20是一种广谱植物生长抑制剂。我们测试了先前表征的CBIs（异恶酰草胺、吲嗪氟草胺和C17）对ES20敏感性降低的拟南芥cesa6突变体的生长的抑制作用。我们发现这些突变体中的大多数对异恶草胺、茚达氟草胺和C17敏感，这表明这些测试的CBI具有与ES 20不同的作用模式。当与其他CBI联合施用时，ES20对植物生长也具有协同抑制作用，进一步证实了ES20具有与异恶酰草胺、吲嗪氟草胺和C17不同的作用模式。我们证明，植物携带两个错义突变赋予ES20和异恶酰草胺的抗性可以耐受这些CBI的双重抑制作用时，组合。ES 20抑制拟南芥在生长培养基和土壤中直接喷洒后的生长。因此，我们的研究结果为使用ES20作为广谱除草剂以及使用基因编辑技术生产抗ES20作物铺平了道路。
Endosidin20 (ES20) was recently identified as a cellulose biosynthesis inhibitor (CBI) that targets the catalytic domain of CELLULOSE SYNTHASE 6 (CESA6) and thus inhibits the growth of Arabidopsis thaliana. Here, we characterized the effects of ES20 on the growth of other plant species and found that ES20 is a broad-spectrum plant growth inhibitor. We tested the inhibitory effects of previously characterized CBIs (isoxaben, indaziflam and C17) on the growth of Arabidopsis cesa6 mutants that have reduced sensitivity to ES20. We found that most of these mutants are sensitive to isoxaben, indaziflam and C17, indicating that these tested CBIs have a different mode of action than ES20. ES20 also has a synergistic inhibitory effect on plant growth when jointly applied with other CBIs, further confirming that ES20 has a different mode of action than isoxaben, indaziflam and C17. We demonstrated that plants carrying two missense mutations conferring resistance to ES20 and isoxaben can tolerate the dual inhibitory effects of these CBIs when combined. ES20 inhibits Arabidopsis growth in growth medium and in soil following direct spraying. Therefore, our results pave the way for using ES20 as a broad-spectrum herbicide, and for the use of gene-editing technologies to produce ES20-resistant crop plants.