Galactosylation at Side Chains of Branched Cyclodextrins by Various β-Galactosidases.
Galactosylation at Side Chains of Branched Cyclodextrins by Various β-Galactosidases.
复制标题
各种 β-半乳糖苷酶对支链环糊精侧链进行半乳糖基化。
DOI：
10.1271/bbb.56.242
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
K. Koizumi
中科院分区：
文献类型：
--
作者：
S. Kitahata;K. Fujita;Y. Takagi;K. Hara;H. Hashimoto;T. Tanimoto;K. Koizumi
以乳糖为供体底物，支链环糊精（CD）为受体，研究了不同β-半乳糖苷酶催化的半乳糖基转移反应。环状芽孢杆菌β-半乳糖苷酶将半乳糖基转移产物合成到支链CD上，其中半乳糖残基连接在支链CD的侧链上，而不是直接连接在CD环上。曲霉属和青霉属的β-半乳糖苷酶也产生在分支CD的侧链处半乳糖基化的衍生物。这些β-半乳糖苷酶对分支CD的主要转半乳糖基化产物的结构似乎与B不同。根据高效液相色谱的保留时间判断，
The galactosyl transfer reaction to branched cyclodextrins (CDs) was investigated using lactose as a donor substrate and branched CDs as acceptors by various β-galactosidases. Bacillus circulans β-galactosidase synthesized galactosyl transfer products to branched CDs, of which the galactose residues were linked at side chains of branched CDs, not directly at CD rings. Aspergillus oryzae and Penicillium multicolor β-galactosidases also produced derivatives galactosylated at side chains of branched CDs. The structures of main transgalactosylation products of branched CDs by these β-galactosidases seem to be different from those by B. circulans β-galactosidase, judging from the retention times on high performance liquid chromatography.