Ribonucleic acid synthesis in regenerating and embryonic liver. I. The synthesis of new species of RNA during regeneration of mouse liver after partial hepatectomy.
Ribonucleic acid synthesis in regenerating and embryonic liver. I. The synthesis of new species of RNA during regeneration of mouse liver after partial hepatectomy.
复制标题
再生和胚胎肝脏中的核糖核酸合成。
DOI：
10.1016/s0022-2836(67)80118-9
复制
发表时间：
1967
影响因子：
5.6
通讯作者：
Brian J. McCarthy
中科院分区：
文献类型：
--
作者：
Robert B. Church;Brian J. McCarthy
RNA与DNA的杂交和其他RNA制剂的竞争效应已被用于分析小鼠部分肝切除后的RNA合成。通过控制实验验证了方法的灵敏度和重复性。在标准RNA/DNA比率下的杂交效率在再生的第一个小时期间增加两倍，并且在7天后下降到正常肝脏的杂交效率，这与部分肝切除术后活性DNA位点数量的急剧增加一致。在正常或假手术动物中不存在的新RNA分子的合成在再生的第一个小时内开始，尽管正常肝脏的RNA合成特征继续进行。通过比较在再生过程中不同时间分离的RNA，可以表明不同分子的合成在不同阶段中断。有些分子在6小时或更晚的时候没有被标记出来，实际上它们基本上不存在;其他种类的RNA在再生的后期消失，直到7天合成的RNA与正常个体的RNA无法区分。肝再生似乎部分由短寿命的RNA分子介导，其合成在部分肝切除术后迅速开始，并在再生过程中的不同时间停止。
Hybridization of RNA with DNA and the competitive effect of other RNA preparations have been used to analyze RNA synthesis following partial hepatectomy in the mouse. Control experiments are presented todemonstrate the sensitivity and repeatability of the methods. The efficiency of hybridization at a standard RNA/DNA ratio increases twofold during the first hour of regeneration and declines to that of normal liver by seven days, consistent with a dramatic increase in the number of active DNA sites following partial hepatectomy. The synthesis of new molecules of RNA not present in normal or sham-operated animals commences within the first hour of regeneration, although the RNA synthesis characteristic of normal liver continues. By comparing RNA isolated at various times during the regeneration process, it can be shown that the synthesis of different molecules is discontinued at various stages. Some of the molecules characteristic of one hour of regeneration are not labeled at six hours or later; indeed they are essentially absent.Other species of RNA disappear at later stages of regeneration, until at seven days the RNA synthesized is not distinguishable from that of normal individuals. Liver regeneration appears to be partly mediated by short-lived RNA molecules, the synthesis of which commences rapidly after partial hepatectomy and ceases at various times during the regenerative process.