Sequence characterized amplified region (SCAR) markers-based rapid molecular typing and identification of Cunninghamia lanceolata
Sequence characterized amplified region (SCAR) markers-based rapid molecular typing and identification of Cunninghamia lanceolata
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DOI：
10.5897/ajb11.2815
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发表时间：
2011-12
影响因子：
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通讯作者：
A. Shen;Haibo Li;Ke Wang;Hongmei Ding;Xin Zhang;Lin-hong Fan;Bo Jiang
中科院分区：
文献类型：
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作者：
A. Shen;Haibo Li;Ke Wang;Hongmei Ding;Xin Zhang;Lin-hong Fan;Bo Jiang
杉木（Cunninghamia lanceolata (Lamb.) Hook.）是我国南方主要用材和造林树种。由于C. lanceolata无性系具有相似的形态特征，因此准确鉴定非常困难。在披针茅无性系选择和育种中，需要一种简单有效的方法来区分这些无性系。然而，迄今为止，C. lanceolata 克隆仅通过传统的表型分析来鉴定。在本研究中，收集了来自不同地理来源的 20 个 C. lanceolata 克隆，并使用随机扩增多态性 DNA (RAPD) 技术进行了分析。克隆并测序了九个多态性 RAPD 带。通过分别从六个测序的多态性 RAPD 带设计六对特异性 SCAR 引物，开发了六个稳定的信息显性序列特征扩增区 (SCAR) 标记。根据20个披针形无性系中检测到的SCAR表型差异，首先从20个研究无性系中鉴定出10个个体无性系，将另外10个无性系分为4个可区分的组。一些来自相同地理来源的 C. lanceolata 无性系也相互区分。该实验是首次将基于SCAR标记的分子方法应用于披针叶茶分子鉴定研究。通过开发并联合使用特异有用的SCAR标记器，完成了披针形无性系的快速分子分型，可作为披针形无性系鉴定的工具。关键词: 杉木, 杉木, 树种选育, SCAR 标记, 分子分型, 鉴定。
Chinese fir ( Cunninghamia lanceolata (Lamb.) Hook.) is the main timber and afforestation tree species in South China. C. lanceolata clones, with similar morphological characteristics, make their exact identification very difficult. A simple and effective method is required to distinguish these clones in the clonal selection and breeding of C. lanceolata . However, C. lanceolata clones have so far been identified only through traditional phenotypic analysis. In the present study, 20 C. lanceolata clones from different geographic provenances were collected and analyzed using randomly amplified polymorphic DNA (RAPD) technique. Nine polymorphic RAPD bands were cloned and sequenced. Six stable informative dominant sequence characterized amplified region (SCAR) markers were developed by designing six pairs of specific SCAR primers from six sequenced polymorphic RAPD bands, respectively. On the basis of the differences in SCAR phenotypes detected among the 20 C. lanceolata clones, 10 individual clones were identified from 20 studied clones at first, and the other 10 clones were divided into 4 distinguishable groups. Some C. lanceolata clones from the same geographic provenances were also distinguished from one another. The experiment was the first report that SCAR markers-based molecular method was used in the research on molecular identification of C. lanceolata . By developing and jointly using specific useful SCAR makers, rapid molecular typing of C. lanceolata clones was accomplished, which could be used as a tool for the identification of C. lanceolata clones. Key words: Chinese fir, Cunninghamia lanceolata , tree breeding, SCAR markers, molecular typing, identification.