Footprinting analysis demonstrates extensive similarity between eukaryotic RNase P and RNase MRP holoenzymes
Footprinting analysis demonstrates extensive similarity between eukaryotic RNase P and RNase MRP holoenzymes
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DOI：
10.1261/rna.1106408
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发表时间：
2008-08-01
期刊：
影响因子：
4.5
通讯作者：
Krasilnikov, Andrey S.
中科院分区：
文献类型：
--
作者：
Esakova, Olga;Perederina, Anna;Krasilnikov, Andrey S.
真核核糖核酸酶（RNase）P和RNase MRP是与进化相关的基于RNA的酶，参与各种RNA分子（包括tRNA和rRNA）的代谢。与由RNA组分和单个小蛋白组成的密切相关的真细菌RNase P相反，这些酶含有多种蛋白组分。在这里，我们报告的足迹研究的结果进行纯化酿酒酵母核糖核酸酶MRP和核糖核酸酶P全酶。结果确定了受蛋白质部分影响的RNA组分的区域，表明蛋白质在稳定RNA折叠中的作用，并指出两种进化相关的基于RNA的酶之间的实质性相似性。
Eukaryotic ribonuclease (RNase) P and RNase MRP are evolutionary related RNA-based enzymes involved in metabolism of various RNA molecules, including tRNA and rRNA. In contrast to the closely related eubacterial RNase P, which is comprised of an RNA component and a single small protein, these enzymes contain multiple protein components. Here we report the results of footprinting studies performed on purified Saccharomyces cerevisiae RNase MRP and RNase P holoenzymes. The results identify regions of the RNA components affected by the protein moiety, suggest a role of the proteins in stabilization of the RNA fold, and point to substantial similarities between the two evolutionary related RNA-based enzymes.