CELLULAR SUBSTRATE OF THE HISTOCHEMICALLY DEFINED STRIOSOME MATRIX SYSTEM OF THE CAUDATE-NUCLEUS - A COMBINED GOLGI AND IMMUNOCYTOCHEMICAL STUDY IN CAT AND FERRET
CELLULAR SUBSTRATE OF THE HISTOCHEMICALLY DEFINED STRIOSOME MATRIX SYSTEM OF THE CAUDATE-NUCLEUS - A COMBINED GOLGI AND IMMUNOCYTOCHEMICAL STUDY IN CAT AND FERRET
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DOI：
10.1016/0306-4522(88)90073-5
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发表时间：
1988-03-01
期刊：
影响因子：
3.3
通讯作者：
GRAYBIEL, AM
中科院分区：
文献类型：
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作者：
BOLAM, JP;IZZO, PN;GRAYBIEL, AM
为了解新纹状体组织化学区段的形态底物，用抗神经肽和递质相关酶的抗血清对猫和雪貂的尾状核和壳核切片进行免疫细胞化学染色，然后进行高尔基体染色。相邻切片染色显示乙酰胆碱酯酶活性，以识别缺乏乙酰胆碱酯酶的纹状体。几种抗体制剂所产生的免疫染色与乙酰胆碱酯酶缺乏的纹状体一致，但这些带中最显著的染色是与P物质直接作用的抗体。纹状体由神经元核膜和树突的P物质免疫染色所描绘，而在吻侧和尾状背核，P物质免疫染色与纹状体外基质之间的边界是陡峭的。出于这些原因，我们在P物质免疫组织化学染色的切片上分析了高尔基体神经元，以评估化学定义的纹状体结构对位于纹状体内和纹状体外基质内的神经元的位置和树突分支的影响。最常见的受孕神经元是中等大小的密集刺状神经元。存在于纹状体内且位于边界一个树突半径内的神经元可分为两类：(1)其树枝分枝明显不受边界影响的神经元；(2)其树枝分枝明显受边界影响的神经元。对于后一种类型的神经元，树突要么从远离边界的核周部分冒出来，从而避免越过边界，要么在路线上表现出突变，显然是为了避免越过边界。位于纹状体外基质中但靠近纹状体边界的刺状神经元具有受或不受间隔边界影响的树突。我们的结论是，新纹状体的组织化学隔间下有一种特殊的细胞结构，不同的中等大小的棘神经元亚群在(1)纹状体和纹状体外基质中信息流的分离和(2)纹状体和纹状体外基质之间的联系下。
In order to learn what morphological substrate might underly the histochemical compartments of the neostriatum, sections of the caudate nucleus and the putamen of cats and ferrets were stained immunocytochemically with antisera directed against several neuropeptides and transmitter-related enzymes and were then Golgi-impregnated. Adjacent sections were stained to reveal acetylcholinesterase activity to identify the acetylcholinesterase-poor striosomes. The immunostaining produced by several of the antibody preparations was in register with the acetylcholinesterase-poor striosomes but the most prominent staining of these zones occurred with the antiodies direct against substance P. The striosomes were delineated by intense substance P-immunostaining of neuronal perikarya and dendrites, and in the rostral and dorsal caudate nucleus the boundary between substance P-immunostained and extrastriosomal matrix was abrupt. For these reasons we analysed Golgi-impregnated neurons in sections immunostained for substance P in order to assess the influence of the chemically defined striosomal architecture on the position and dendritic arborization of neurons located both within the striosomes and within the extrastriosomal matrix. The most commonly impregnated neurons were of the medium-size densely spiny class. Those that were present within the striosomes and lay within one dendritic radius of the boundary were divided into two types: (1) neurons whose dendritic arborization was apparently not influenced by the boundary and (2) neurons whose dendritic arborization was markedly influenced by the boundary. For neurons of the latter type, dendrities either emerged from the parts of the perikaryon away from the boundary, so avoiding crossing it, or they exhibited abrupt changes in their course, apparently to avoid crossing the boundary. Spiny neurons located in the extrastriosomal matrix but close to the striosomal boundary had dendrites that were either influenced by, or not influenced by the compartmental boundary. We conclude that there is a specific cytoarchitecture underlying the histochemical compartments of the neostriatum and that different sub-populations of medium-size spiny neurons underly (1) the segregation of information flow in striosomes and the extrastriosomal matrix and (2) communication between striosomes and the extrastriosomal matrix.