Protective effects of memantine and epicatechin on catechol-induced toxicity on Muller cells in vitro
Protective effects of memantine and epicatechin on catechol-induced toxicity on Muller cells in vitro
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DOI：
10.1016/j.tox.2010.03.013
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发表时间：
2010-05-27
期刊：
影响因子：
4.5
通讯作者：
Kenney, M. Cristina
中科院分区：
文献类型：
--
作者：
Mansoor, Saffar;Gupta, Navin;Kenney, M. Cristina
This study evaluates the toxic effects of catechol (a component from cigarette smoke) on Muller cells (MIO-M1) in vitro, and investigates the inhibitors memantine and epicatechin to determine if they can reverse the catechol toxic effects. MIO-MI cells were exposed to varying concentrations of catechol with or without memantine or epicatechin. Cell viability (CV) was measured by a trypan blue dye-exclusion assay. Caspase-3/7 activity was measured by fluorochrome assay. The production of reactive oxygen/nitrogen species (ROS/RNS) was measured with 2',7'-dichlorodihydrofluorescein diacetate dye assay. Mitochondrial membrane potential (Delta Psi m) was measured using JC-1 assay. Intracellular ATP content was determined by the ATPLite kit. MIO-M1 cells showed significant decrease in cell viability, increased caspase-3/7 activity, elevated ROS/RNS levels, decreased Delta Psi m value, and decreased intracellular ATP content after exposure to catechol 150, 300, and 600 mu M compared with control. Pre-treatment with memantine 10 mu M or epicatechin 15 mu M reversed loss of cell viability in catechol 150 mu M-treated cultures (22.3%, p
