Synthetic promoter libraries - tuning of gene expression
Synthetic promoter libraries - tuning of gene expression
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DOI：
10.1016/j.tibtech.2005.12.003
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发表时间：
2006-02-01
影响因子：
17.3
通讯作者：
Jensen, PR
中科院分区：
文献类型：
--
作者：
Hammer, K;Mijakovic, I;Jensen, PR
基因功能的研究通常需要改变基因的表达并评估其后果。原则上，任何给定基因的表达都可以在离散表达水平的准连续体中进行调节，但传统方法通常限于两个极端：基因敲除和强过表达。然而，代谢优化和对照分析等应用需要一组连续的表达水平，其强度仅略有增加，以覆盖所研究基因的野生型表达水平周围的特定窗口;这一要求可以通过使用启动子文库来满足。这种方法通常包括在待研究的基因前插入启动子文库，由此单个启动子可能在其间隔序列中偏离或与营养启动子的共有序列有轻微偏离。在这里，我们描述了两种不同的方法获得启动子库，并比较其适用性。
The study of gene function often requires changing the expression of a gene and evaluating the consequences. In principle, the expression of any given gene can be modulated in a quasi-continuum of discrete expression levels but the traditional approaches are usually limited to two extremes: gene knockout and strong overexpression. However, applications such as metabolic optimization and control analysis necessitate a continuous set of expression levels with only slight increments in strength to cover a specific window around the wildtype expression level of the studied gene; this requirement can be met by using promoter libraries. This approach generally consists of inserting a library of promoters in front of the gene to be studied, whereby the individual promoters might deviate either in their spacer sequences or bear slight deviations from the consensus sequence of a vegetative promoter. Here, we describe the two different methods for obtaining promoter libraries and compare their applicability.