Comprehensive observation of trichome development in Micro-tom tomato
Comprehensive observation of trichome development in Micro-tom tomato
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DOI：
10.48130/vr-2021-0006
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Jiang Chang;Jie Yang;Jianfeng Wang;Shuang Wu
中科院分区：
文献类型：
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作者：
Jiang Chang;Jie Yang;Jianfeng Wang;Shuang Wu
毛状体通过形成保护屏障帮助植物抵御各种压力。虽然拟南芥的单细胞毛状体已经得到了很好的研究，但对番茄多细胞毛状体的发育知之甚少。番茄的毛状体有七种形态和功能上不同的类型，比拟南芥的单细胞毛状体更复杂。本文对番茄表皮毛发育的时空格局进行了综合分析。我们的观察表明，在叶发育过程中，I/IV型毛状体的逐渐减少是伴随着其他毛状体类型的增加。我们通过构建在毛状体的顶细胞中特异性表达的标记报告基因pSlAT 2：GUS-YFP进一步研究了I/IV型毛状体的腺体发育。有趣的是，我们发现毛状体的多细胞基础早在两细胞阶段就开始了。通过对番茄表皮毛细胞核标记H2 B-GFP的追踪，发现柄细胞长度与核大小之间存在相关性，提示细胞倍性水平可能影响表皮毛细胞大小。最后，我们发现VII型腺毛表现出两种形态上不同的形式，大部分被蜡样物质覆盖，这可能有助于形成额外的储存空间，用于分泌代谢物。本研究结果为进一步研究番茄毛状体发育机理奠定了基础。
Trichomes help plants defend against various stresses by formation of a protective barrier. Although the unicellular trichomes in Arabidopsis have been well studied, little is known of the development of multicellular trichomes in tomato. With seven morphologically and functionally different types, tomato trichomes are more complex than the unicellular trichomes in Arabidopsis. Here, we comprehensively analyzed the spatiotemporal pattern of tomato trichome development. Our observations show that the gradual reduction of type I/IV trichomes during leaf development is accompanied by an increase of other trichome types. We further examined the gland development of type I/IV trichomes by constructing a marker reporter, pSlAT2: GUS-YFP which is specifically expressed in the top cell of the trichomes. Interestingly, we found the multicellular base of trichomes is initiated as early as the two-cell stage. Through tracking a nuclear marker, H2B-GFP in tomato trichomes, we found the correlation between stalk cell length and nuclear size, suggesting that cell ploidy level could affect trichome cell size. Finally, we found type VII glandular trichomes exhibit two morphologically distinct forms, with the majority covered by a wax-like substance, which could help to form extra storage space for secretory metabolites. Our results provide the basis for further mechanistic study of trichome development in tomato.