Identification of functional human splenic memory B cells by expression of CD148 and CD27.
Identification of functional human splenic memory B cells by expression of CD148 and CD27.
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DOI：
10.1084/jem.188.9.1691
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发表时间：
1998-11-02
期刊：
影响因子：
--
通讯作者：
de Vries JE
中科院分区：
文献类型：
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作者：
Tangye SG;Liu YJ;Aversa G;Phillips JH;de Vries JE
从人扁桃体分离的记忆B细胞的特征在于活化的细胞表面表型、定位于粘膜上皮、体细胞突变的免疫球蛋白（IG）可变（V）区基因的表达以及体外优先分化为浆细胞。在人类和啮齿类动物的脾脏中，记忆B细胞的一个亚群被认为位于白色髓的边缘区。与扁桃体来源的记忆B细胞相似，脾边缘区B细胞可通过不同的细胞表面表型和其IG V区基因中存在体细胞突变而与幼稚滤泡B细胞区分开。尽管人类幼稚和记忆B细胞之间存在差异，但尚未鉴定出阳性鉴定所有记忆B细胞的细胞表面分子。在这项研究中，我们研究了受体型蛋白酪氨酸磷酸酶CD 148对人类B细胞的表达。存在于人脾脏中的CD 148 + B细胞表现出记忆B细胞的典型特征。这些包括活化的表型，定位于边缘区，体细胞突变的IG V区基因的表达，以及优先分化为浆细胞。相比之下，CD 148 − B细胞似乎是幼稚B细胞，这是由于其定位于套层，表达未受刺激的B细胞的典型表面抗原，以及表达未突变的IG V区基因。有趣的是，CD 148 + B细胞也共表达CD 27，而CD 148 − B细胞是CD 27 −。这些结果将CD 148和CD 27鉴定为阳性鉴定人脾中存在的记忆B细胞的标志物。因此，评估这些分子的表达可能是监测免疫受损个体或疫苗试验中记忆B细胞应答的发展的方便方法。
Memory B cells isolated from human tonsils are characterized by an activated cell surface phenotype, localization to mucosal epithelium, expression of somatically mutated immunoglobulin (Ig) variable (V) region genes, and a preferential differentiation into plasma cells in vitro. In spleens of both humans and rodents, a subset of memory B cells is believed to reside in the marginal zone of the white pulp. Similar to tonsil-derived memory B cells, splenic marginal zone B cells can be distinguished from naive follicular B cells by a distinct cell surface phenotype and by the presence of somatic mutations in their Ig V region genes. Although differences exist between human naive and memory B cells, no cell surface molecules have been identified that positively identify all memory B cells. In this study, we have examined the expression of the receptor-type protein tyrosine phosphatase CD148 on human B cells. CD148+ B cells present in human spleen exhibited characteristics typical of memory B cells. These included an activated phenotype, localization to the marginal zone, the expression of somatically mutated Ig V region genes, and the preferential differentiation into plasma cells. In contrast, CD148− B cells appeared to be naive B cells due to localization to the mantle zone, the expression of surface antigens typical of unstimulated B cells, and the expression of unmutated Ig V region genes. Interestingly, CD148+ B cells also coexpressed CD27, whereas CD148− B cells were CD27−. These results identify CD148 and CD27 as markers which positively identify memory B cells present in human spleen. Thus, assessing expression of these molecules may be a convenient way to monitor the development of memory B cell responses in immunocompromised individuals or in vaccine trials.