Electronic cigarettes increase endothelial progenitor cells in the blood of healthy volunteers
Electronic cigarettes increase endothelial progenitor cells in the blood of healthy volunteers
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DOI：
10.1016/j.atherosclerosis.2016.09.064
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发表时间：
2016-12-01
期刊：
影响因子：
5.3
通讯作者：
Lundback, Magnus
中科院分区：
文献类型：
--
作者：
Antoniewicz, Lukasz;Bosson, Jenny A.;Lundback, Magnus
背景和目的：电子烟的使用在全球范围内急剧增加，其对人类健康的影响仍不确定。在本研究中，我们测定了健康青年志愿者短期吸入电子烟蒸气(ECV)后内皮祖细胞(EPC)和微囊泡(MVS)的变化，以确定血管的变化。方法：16名健康的不吸烟者按交叉设计随机分为两组，暴露于10支ECV 10分钟和不吸入ECV 10分钟。分别于染毒前、染毒后1、4、24小时采血。用流式细胞仪分析内皮祖细胞(CD34+CD309)和巨噬细胞集落刺激因子(MVS)。根据来源(血小板(CD41)、内皮细胞(CD144)、白细胞(CD45)、单核细胞(CD14))和核含量(Syto 13染料)对MV进行表型分析。检测炎症标志物P-选择素(CD62P)、E-选择素(CD62E)、CD40-配体(CD154)和HMGB1的表达。结果：体外循环暴露后1h血中内皮祖细胞水平显著升高，24 h后恢复至基础水平，仅E-选择素阳性的内皮来源血管内皮细胞轻微升高(p<；0.038)。FeNO不受ECV暴露的影响。结论：在健康志愿者中，吸入10次电子烟蒸气可导致内皮祖细胞增加。正如我们之前所展示的，这一增加的幅度与吸烟一支传统香烟的幅度相同。综上所述，这些结果可能代表了短暂吸入电子烟后血管可能发生变化的迹象。随着电子烟市场的持续发展，分析潜在的心血管健康影响的进一步研究至关重要。(C)2016爱思唯尔爱尔兰有限公司。保留所有权利。
Background and aims: The use of electronic cigarettes is increasing dramatically on a global scale and its effects on human health remain uncertain. In the present study, we measured endothelial progenitor cells (EPCs) and microvesicles (MVs) in healthy young volunteers following short-term exposure to inhalation of e-cigarette vapor (ECV) to determine vascular changes.Methods: Sixteen healthy seldom smokers were randomized into two groups either exposed or not exposed to 10 puffs of ECV for 10 min, in a crossover design. Blood samples were obtained at baseline and 1, 4 and 24 h following exposure. EPCs (CD34 + CD309) and MVs were analyzed by flow cytometry. MVs were phenotyped according to origin (platelet (CD41), endothelial (CD144), leukocytes (CD45), monocytes (CD14)) and nuclear content (SYTO 13 dye). In addition, expression of inflammation markers such P-selectin (CD62P), E-selectin (CD62E), CD40-ligand (CD154) and HMGB1 was investigated. Fractional exhaled nitric oxide (FeNO) was also measured at baseline and after 24 h.Results: EPC levels in blood were significantly increased 1 h following exposure to ECV and returned to baseline values after 24 h. Only E-selectin positive MVs (endothelial origin) were slightly elevated (p < 0.038). FeNO was unaffected by exposure to ECV.Conclusions: In healthy volunteers, ten puffs of e-cigarette vapor inhalation caused an increase in EPCs. This increase was of the same magnitude as following smoking of one traditional cigarette, as we previously demonstrated. Taken together, these results may represent signs of possible vascular changes after short e-cigarette inhalation. Further studies analyzing potential cardiovascular health effects are critical as the e-cigarette market continues to burgeon. (C) 2016 Elsevier Ireland Ltd. All rights reserved.